Cloning and expression of rhl AB operon under the control of tac promoter in E. coli

سال انتشار: 1396
محل انتشار: مجله بین المللی میکروبیولوژی مولکولی و بالینی، دوره: 7، شماره: 2
کد COI اختصاصی: JR_IJMCM-7-2_006
زبان مقاله: انگلیسی
تعداد مشاهده: 36

نویسندگان

National Institute of Genetics and Biotechnology Engineering, Research Institute of Medical Biotechnology, Department of Molecular Medicine

seyed abolghassem mohammadi bondarkhilli

National Institute of Genetics and Biotechnology Engineering, Research Institute of Medical Biotechnology, Department of Molecular Medicine

Mehdi MohMMADI

Natinal Institute of Genetic Engineering and Biotechnology

Kambiz Akbari

Natinal Institute of Genetic Engineering and Biotechnology

Hossein Shahbani

National Institute of Genetic Engineering and Biotechnology

Arefeh Alipour

National Institute of Genetic Rngineering and Biotechnology

چکیده

Today, efforts go towards the replacement of chemical surfactants by natural biological biosurfactants (biosurfactant), as these materials are not carcinogenic and highly compatibile with the environment. One of the main classes of biosurfactants is rhamnose containing glycolipid biosurfactant (rhamnolipids). This type of biosurfactants can be applied in many industries such as oil industry, pharmaceutical industry, food industry and esc. In the present study, with elimination of regulatory elements of monorhamnolipid operon, the suitable construct was made and transformed to E. coli BL۱۲ and E. coli DH۵α competent cells. Then the production of monorhamnolipid in recombinant E. coli BL۱۲ harboring pET ۱۲a vector and mono and di-rhamnolipid biosurfactant in recombinant E. coli DH۵α harboring pTrc ۹۹A evaluated by CTAB plate and thin layer chromatography (TLC). The relative production of mono and dirhamnolipid biosurfactant was determined by oil spreading method. Finally, the efficiency of the extracted biosurfactant was verified by E۱۲ method in different salinity and pH conditions.

کلیدواژه ها

Biosurfactant, Rhamnolipid, E. coli BL۱۲, E. coli DH۵α, transformation

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.