Isolation and characterization of bacterial strains from shoreline waters of Caspian Sea with novel antibacterial activity
- سال انتشار: 1397
- محل انتشار: مجله بین المللی میکروبیولوژی مولکولی و بالینی، دوره: 8، شماره: 2
- کد COI اختصاصی: JR_IJMCM-8-2_001
- زبان مقاله: انگلیسی
- تعداد مشاهده: 35
نویسندگان
Department of Microbiology, Faculty of Science, Islamic Azad University-Lahijan Branch,
Department of Microbiology, Faculty of Science, Islamic Azad University-Lahijan Branch,
Persian Type Culture Collection, Iranian Organization for Science and Technology, Tehran, Iran
Microbiology, Faculty of Basic Sciences, Islamic Azad University-Lahijan Branch
چکیده
In this study, a total ۱۰۸ bacterial strains with potential of antibacterial activity were isolated from ۱۴۵ shoreline water samples of Caspian Sea. The isolated bacteria were: Gram negative and positive bacilli, Gram negative and positive cocci and Gram negative coccoid. Amongst ۱۰۸ isolated bacterial strains, ۱۲ strains (۱۱%) showed significant antibacterial activity against ۱۰ reference strains. Morphological, physiological, biochemical features and ۱۶S rRNA analysis were used to identify the isolated strains with antibacterial activity. Amongst the isolated strains, six isolates possessed typical cellular, colonial morphologies, physiological, biochemical and nutritional features that resembled Bacillus spp. Four strains were identified as Bacillus cereus. The two other isolates belonged to Bacillus subtilis group. Two gram positive rod shape isolates were identified as Exiguobacterium acetylicum. The rest of isolates with antibacterial potency were gram negative bacteria. They were identified as Pseudomonas aeruginosa (۳isolate) and Alcaligenes faecalis (one isolate). Crude extracts of Pseudomonas aeruginosa strains showed significant antimicrobial activity against all reference strains. Also, E.acetylicum and A. faecalis showed antibacterial activity against some reference strains such as Escherichia coli PTCC ۱۵۳۳, Shigella flexneri PTCC ۱۲۳۴ and Salmonella enterica subsp.enterica Paratyphi B PTCC ۱۲۳۱. The crude extracts of four isolates with wide antibacterial activity were used in TLC autobiography overlay assay. The TLC autobiographic overlay assay implied that the antimicrobial metabolites produced by the strains with wide antimicrobial spectrum were different.کلیدواژه ها
Caspian Sea, antibacterial activity, isolation, marine bacteria, Characterizationاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.