Study of Anti-Retroviral Effects of Salix Aegyptiaca L Herbal Extract on HIV-۱ in-vitro
- سال انتشار: 1393
- محل انتشار: مجله بین المللی میکروبیولوژی مولکولی و بالینی، دوره: 4، شماره: 1
- کد COI اختصاصی: JR_IJMCM-4-1_010
- زبان مقاله: انگلیسی
- تعداد مشاهده: 132
نویسندگان
Department of Biochemistry, College of Basic Sciences, Tehran, Science and Research Branch, Islamic Azad University, Tehran, Iran
Department of Chemistry, College of Basic Sciences, Tehran, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Chemistry, College of Basic Sciences, Tehran, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Biology, College of Basic Sciences, Tehran, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده
Human immunodeficiency virus (HIV) infection is the major problem in the world. Nowadays, new anti-retroviral drugs are extracted from medicinal plants, make an important role to supersede synthetic drugs with different side effects. In this study, we studied the Salix aegyptiaca L extract, Iranian herb, as an anti-HIV drug with anti-retroviral effects. We used a single cycle replicable (SCR) HIV-۱ system by co-transfection of HEK ۲۹۳T with pmzNL۴-۳, psPAX۲ and pM۲G.۲. Cytotoxicity and cytopathic protection assay were performed by XTT method. Inhibition of p۲۴ Ag production level assay was done using quantitative enzyme linked immunosorbent assay (ELISA). Salix aegyptiaca L inhibited HIV-۱ induced CPE in HELA cells with SI value of ۲۲.۲. Finally, we use informatics analysis such as Gaussian, Hyper Chem , HF and B۳LYP methods and ۶-۳۱G, ۶-۳۱G** and ۶-۳۱۱G** basis sets. These results suggest that Salix aegyptiaca L has potent anti-HIV activity and may be a promising candidate for AIDS.کلیدواژه ها
Human immunodeficiency virus۱, Salix aegyptiaca L, single cycle replicable virion (SCR), Gaussian, HF, Hyper Chemاطلاعات بیشتر در مورد COI
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