Sara Amini - Bayer School of Natural and Environmental Sciences, Duquesne University, Pittsburgh, PA, USA
Amir Hossein Omidi - Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran
Hamed Afkhami - Department of Medical Bacteriology, Faculty of Medicine, Shahed University, Tehran, Iran
Hoda Sabati - Biotechnology and Biological Science Research Center, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Amin Mohsenzadeh - Tehran Heart Center, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran
Atyieh Soleymani - Department of Molecular and Cellular Sciences, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Mohammad Ali Zonobian - Department of Food Microbiology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
Negin Ghanbarnejad - Department of Biotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Mohammad Reza Mohammadi - Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Staphylococcus aureus is one of the most common infectious bacterial species and one of the agents of community-acquired infections (CAIs) and hospital-acquired infections (HAIs). Aminoglycosides are potent antibactericidal agents often used together with Beta Lactams or Glycopeptides, especially in treating Staphylococcal endocarditis. The present research aimed to determine the frequency of the aac (۶ ́)-le-aph (۲ ́ ́) gene that encodes Aminoglycoside modifying enzymes using PCR on clinical isolates of S. aureus. ۱۱۵ clinical isolates of S. aureus were collected at educational hospitals in Karaj during ۱۲ months. They were first identified by using standard biochemical and laboratory methods and, following CLSI principles and procedures, antibiotic sensitivity patterns of all isolates were obtained using the disc diffusion method. Moreover, using agar dilution, the minimum inhibitory concentration was determined using the antibiotic powder Gentamycin. Finally, gene frequency was measured by employing PCR. The highest levels of resistance to Aminoglycosides were observed in Kanamycin (۴۷.۸%), Gentamycin (۴۶.۹%), and Tobramycin (۴۶.۹%), and Doxycycline and Ciprofloxacin with ۵۰.۴ and ۴۹.۵ percent respectively, were the non-Aminoglycoside antibiotics to which the highest levels of resistance were exhibited. The frequency of the aac (۶ ́)-le-aph (۲ ́ ́) gene was ۳۹.۱ percent. Rapid and timely detection of resistant strains seems to be necessary in selecting suitable treatment options and in preventing the spread of resistance. Furthermore, rapid identification of genes that encode AME enzymes using PCR enjoys special advantages such as high levels of precision and speed.

AME enzymes, Beta Lactams, Deep Abscesses, Endocarditis, Osteomyelitis, Pneumonia, Sepsis