Frequency of aac (۶ ́)-le-aph (۲ ́ ́) gene and resistance to Aminoglycoside antibiotics in Staphylococcus aureus isolates
- سال انتشار: 1403
- محل انتشار: مجله تحقیقات سلولی، مولکولی و زیست پزشکی، دوره: 4، شماره: 2
- کد COI اختصاصی: JR_CMBR-4-2_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 84
نویسندگان
Bayer School of Natural and Environmental Sciences, Duquesne University, Pittsburgh, PA, USA
Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran
Department of Medical Bacteriology, Faculty of Medicine, Shahed University, Tehran, Iran
Biotechnology and Biological Science Research Center, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Tehran Heart Center, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran
Department of Molecular and Cellular Sciences, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Department of Food Microbiology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
Department of Biotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
چکیده
Staphylococcus aureus is one of the most common infectious bacterial species and one of the agents of community-acquired infections (CAIs) and hospital-acquired infections (HAIs). Aminoglycosides are potent antibactericidal agents often used together with Beta Lactams or Glycopeptides, especially in treating Staphylococcal endocarditis. The present research aimed to determine the frequency of the aac (۶ ́)-le-aph (۲ ́ ́) gene that encodes Aminoglycoside modifying enzymes using PCR on clinical isolates of S. aureus. ۱۱۵ clinical isolates of S. aureus were collected at educational hospitals in Karaj during ۱۲ months. They were first identified by using standard biochemical and laboratory methods and, following CLSI principles and procedures, antibiotic sensitivity patterns of all isolates were obtained using the disc diffusion method. Moreover, using agar dilution, the minimum inhibitory concentration was determined using the antibiotic powder Gentamycin. Finally, gene frequency was measured by employing PCR. The highest levels of resistance to Aminoglycosides were observed in Kanamycin (۴۷.۸%), Gentamycin (۴۶.۹%), and Tobramycin (۴۶.۹%), and Doxycycline and Ciprofloxacin with ۵۰.۴ and ۴۹.۵ percent respectively, were the non-Aminoglycoside antibiotics to which the highest levels of resistance were exhibited. The frequency of the aac (۶ ́)-le-aph (۲ ́ ́) gene was ۳۹.۱ percent. Rapid and timely detection of resistant strains seems to be necessary in selecting suitable treatment options and in preventing the spread of resistance. Furthermore, rapid identification of genes that encode AME enzymes using PCR enjoys special advantages such as high levels of precision and speed.کلیدواژه ها
AME enzymes, Beta Lactams, Deep Abscesses, Endocarditis, Osteomyelitis, Pneumonia, Sepsisاطلاعات بیشتر در مورد COI
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