The Relationship of Toxoplasma Gondii Infection with Men Infertility: A Serological and Molecular Study
- سال انتشار: 1402
- محل انتشار: مجله بین المللی آزمایشگاه پزشکی، دوره: 10، شماره: 4
- کد COI اختصاصی: JR_JIML-10-4_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 79
نویسندگان
Cellular & Molecular Research Center, Basic Health Sciences Institute Shahrekord University of Medical Sciences, Shahrekord, Iran.
Modeling in Health Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Cellular & Molecular Research Center, Basic Health Sciences Institute Shahrekord University of Medical Sciences, Shahrekord, Iran
Department of Immunology and Allergy, ACECR, Tehran, Iran
Cellular & Molecular Research Center, Basic Health Sciences Institute Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Obstetrics and Gynecology, Shahrekord University of Medical Science, Shahrekord, Iran
چکیده
Background and Aims: Toxoplasma gondii (T. gondii) is one of the most dangerous parasitic infections in immunocompromised patients. It may pass through the bloodstream to various organs, such as the reproductive organs of intermediate hosts, which may lead to male infertility. Therefore, this study aimed to determine the relationship between T. gondii infection and infertility in infertile men referred to the infertility center of Chaharmahal and Bakhtiari province. Materials and Methods: In this case-control study,۱۲۹ men, including ۶۷ infertile and ۶۲ fertile men, participated, and specific anti-Toxoplasma antibodies of IgG and IgM were detected in the serum of all cases using enzyme-linked immunosorbent assay methods. Also, the presence of T.gondii DNA in the seminal fluid samples of these people was detected using the polymerase chain reaction method. Sperm analyses, including sperm count, morphology, motility, and viscosity, were also performed on the seminal fluid. Results: Based on the result of the IgG antibody, a significant difference was found in toxoplasmosis in two fertile and infertile groups. Its frequency was ۱۶.۱% in the fertile group and ۴۰.۳% in the infertile group. Also, Toxoplasma infection showed a relationship with sperm count and sperm morphology, so its frequency was ۷۲.۷% in the low sperm group and ۲۴.۶% in the high sperm group. The results of IgM and polymerase chain reaction showed, there was no significant relationship between the fertile and infertile groups with toxoplasmosis. Conclusion: The serum prevalence of the anti-Toxoplasma antibodies and molecular detection in infertile men is higher than the control group, and it seems that Toxoplasma may be an effective risk factor for causing male infertility and disruption of sperm parameters.کلیدواژه ها
Infertility, Men, PCR, Toxoplasma gondiiاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.