Antibacterial and Healing Effect of Silver Nanoparticles Derived from Nettle Extracts on Burn Infections of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa

BACKGROUND AND ABJECTIVES. aureus, E. coli and P. aeruginosa are common causes of burn infections and illnesses. Silver nanoparticles have many applications, one of the necessary applications is antimicrobial and healing effect. There are various methods for synthesis like green synthesis which is more efficiency and eco-friendly. In this study, antibacterial and healing effect of silver nanoparticles derived from Urtica dioica on burn infections of S. aureus, E. coli and P. aeruginosa has been reported.MATERIALS AND METHODSIn this study, acetonic extracts of Urtica dioica prepared with soaking method. The extract was added to ۰.۱ M of silver nitrate solution for biosynthesing of Ag+ nanoparticles. Efficiency of Ag+ synthesis was confirmed by XRD and DLS technique. The MBC and MIC of the extracts and nanoparticles were determined with microdilution method. In animal model, bacteria were inoculated with a concentration of (۵×۱۰۵ CFU/ml) to the wound side of rats. After ۲۴ hours ointments were prepared based on MBC concentration of extracts and nanoparticles with ۱g Eucerin for treat burn wounds and infections.RESULTS AND DISCUSSIONAnimal studies shows that silver nanoparticles synthesized by Urtica dioica are effective on S. aureus, E. coli and P. aeruginosa. But acetonic extract of mentioned plant is effective on S.aureus and E.coli. As a result, silver nanoparticles derived by extracts have more antimicrobial and healing effect than acetonic extracts. The wound which treated with nanoparticles seen healing activity.CONCLUSIONAcetonic extracts of mentioned plant and silver nanoparticles obtained by Urtica dioica have antimicrobial and healing activity in low concentration on S. aureus, E. coli and P. aeruginosa. But acetonic extract of Urtica dioica have healing and antimicrobial activity in low concentration on S. aureus, E. coli and effective in high concentration on P. aeruginosa.

کلیدواژه ها:

Escherichia coli ، silver ، Staphylococcus aureus ، Pseudomonas aeruginosa ، Urtica dioica.