Identification of some genes associated with virulence and antibiotic resistance in Campylobacter jejuni by using bioinformatics tools
- سال انتشار: 1402
- محل انتشار: بیست و چهارمین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM24_622
- زبان مقاله: انگلیسی
- تعداد مشاهده: 229
نویسندگان
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Department of Bacteriology, Tarbiat Modares University, Tehran, Iran
چکیده
BACKGROUND AND OBJECTIVESCampylobacter jejuni, a zoonotic pathogen, is widely acknowledged as a significant contributor to global foodborne bacterial illnesses. In addition, there is growing evidence that Guillain-Barré syndrome, an inflammatory demyelinating disease of the peripheral nervous system, is frequently preceded by C.jejuni infection. Virulence and antibiotic resistance can manifest in various adaptive, innate, and acquired forms. The intricate regulation of genes responsible for both antibiotic resistance and virulence is interconnected and influenced by environmental factors, posing challenges for gene regulation. Key mechanisms involved in regulation establish a connection between antibiotic resistance and virulence. This study aimed to investigate the expression of virulence genes and antibiotic resistance determinants in C.jejuni, by using bioinformatics analysis as well as to investigate the relationship between these two traits.MATERIALS AND METHODSThe microarray data with the accession IDs GSE۲۸۲۹۵ and GSE۱۷۸۸۱ were identified, extracted and analyzed by GEO۲R online tools and R software. Genes with the highest differential expression were identified using parameters P< ۰.۰۵ and LogFC> |۱|. Subsequently, the expression of the related genes was isolated and for the genes that had an increase in expression, the protein network was predicted by STRING database and visualized with the Cytoscape software.RESULTS AND DISCUSSIONBy identifying different genes that had a significant decrease or increase in expression, it was found that a set of genes (acnB, gltA, sdhA, sdhB) are involved in virulence, while another set of genes (flgB, CmeC, sdhA, sdhB) may contribute to the antibiotic resistance of C.jejuni. Additionally, a relationship between sdhA and sdhB genes in both virulence and antibiotic resistance to Erythromycin was revealed.CONCLUSIONThe findings of this study by utilizing bioinformatics tools, highlight the complexity of therelationship between virulence and antibiotic resistance in C. jejuni, which has become a major concern to the emergence of multidrug-resistant strains. Further investigation is needed to better understand Campylobacter pathogenesis, with an approach considering the interactions between virulence markers and antibacterial resistance.کلیدواژه ها
Campylobacter jejuni, antibiotic resistance, virulence, microarray, bioinformatics analysis.مقالات مرتبط جدید
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