High Prevalence of Extended Spectrum β-lactamases producing Escherichia coli in Patients with Urinary tract infection in Hamedan, Iran
- سال انتشار: 1402
- محل انتشار: بیست و چهارمین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM24_529
- زبان مقاله: انگلیسی
- تعداد مشاهده: 60
نویسندگان
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.
Department of Obstetrics and Gynaecology, Iranian Social Security Organization, Atiyeh Hospital, Hamedan, Iran
چکیده
BACKGROUND AND ABJECTIVEUrinary tract infection (UTI) is one of the most common infections in humans which probably affects one-half of all people during their lifetimes. Uropathogenic Escherichia coli (UPEC), one of the members of the extra-intestinal pathogenic E. coli (ExPEC), is the main etiologic agent of UTI. The excessive and inappropriate use of antimicrobial agents specially β-lactam antibiotics for treatment of UTI infections has recently led to the emergence of multiple drug resistance (MDR) and increasing in Extended Spectrum β-lactamases (ESBL) producing E. coli, worldwide. The present study was conducted to investigate the presence of ESBLs among the UPESs isolated from patients with UTI referred to hospitals in Hamedan city using phenotypic and molecular methods.MATERIALS AND METHODSA total of ۱۰۰ UPEC isolates, were used in this study. These strains were isolated from the patients with UTI referred to hospitals in Hamedan city, Hamedan, Iran. Bacterial isolation and identification was done using conventional microbiology methods and standard biochemical tests, additional identification was made using molecular methods. The UPEC isolates were screened for ESBLs production by the double-disk synergy test using Ceftazidime (۳۰ μg) and Cefotaxime (۳۰ μg) disks and confirmed by combined disk diffusion test using Clavulanic acid according to the guidelines recommended by the Clinical and Laboratory Standards Institute (CLSI, ۲۰۲۱). The presence of four β-lactamases gene including blaTEM, blaSHV, blaOXA, and blaCTX-M among ESBLs producer UPEC isolates (n= ۴۰) was investigated using PCRRESULTS AND DISCUSSIONFrom a total of ۱۰۰ UPEC isolates, ۴۰ (۴۰%) isolates were phenotypically identified as ESBLs producers. Among ۴۰ ESBLs producers UPEC isolate the most prevalent β-lactamases gene was blaCTX-M ۱۹/۴۰ (۴۷.۵%) followed by blaTEM ۲۲.۵% (۹/۴۰) and blaOXA ۲۰ % (۸/۴۰). The blaSHV gene was not found in any tested isolates.CONCLUSIONDue to relatively high prevalence of ESBLs producer UPEC isolates in patients with UTI and incidence of β-lactamases gene among these isolate, applying precise antibiotic stewardship, continuous surveillance in order to use appropriate antibiotics, and the control of UTI infections seem necessary to decrease ESBLs producer UPEC.کلیدواژه ها
Extended Spectrum β-lactamases (ESBL), Uropathogenic Escherichia coli (UPEC), Urinary tract infection (UTI), β-lactamases gene, PCRمقالات مرتبط جدید
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