Evaluation of expression systems utilized in monoclonal antibody production
- سال انتشار: 1402
- محل انتشار: بیست و چهارمین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM24_506
- زبان مقاله: انگلیسی
- تعداد مشاهده: 87
نویسندگان
Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
چکیده
BACKGROUND AND OBJECTIVESMonoclonal antibodies (mAbs) are innovative and promising tools utilized in targeting the desired epitope of a specific antigen with highest affinity in order to enhance the functionality of immune system in response to potential immunogens. The aim of this review is to evaluate the proficiency of hosts utilized in mAb production.MATERIALS AND METHODSTo conduct this research, we studied ۳۲ research articles regarding mAb manufacture on PubMed and Google Scholar.RESULTS AND DISCUSSIONHybridoma technology is the primitive and favored method employed in production of mAbs. Selection of an appropriate host is contingent on various factors; existence of a homologous protein in immunized species, compatibility of the protein with human body and intensity of induced immune response are all vital factors in this process. BALB/c is the most frequent mammalian host used in production of mAbs due to the phylogenetic similarities to humans. Rabbits are alternative competent hosts utilized in mAb production due to offering superior characteristics such as ۱۰x to ۱۰۰x more affinity comparing to mice. Despite the commendable attempts to tackle the lack of myeloma cell line in this host with inducing viral transformation, genetic instability of cell line has led to limited success. Utilization of human hosts might seem to be the most convenient approach as they eliminate the necessity of molecular post-modifications and the products will be highly compatible. However, limited number of fusion partners and low efficiency of human cells unveil further challenges. Yeasts, insects and plants are substitute expression systems utilized in mAb manufacture. Phage display is an alternative and auspicious technique used for expression of desired mAb on surface protein of a bacteriophage as it provides a vast number of potent expression systems. Yet, folding problems of the bioproducts and limited expression capability of viruses deliver novel obstacles to resolve.CONCLUSIONMAbs acquire appreciable utility as well as apparent issues to be addressed. Selection of a well-qualified host in the production process plays a vital role in maximizing the efficiency of manufacture. Propitious outcomes of mAb applications in combination with additional therapeutics, encourage further studies in this regard.کلیدواژه ها
Monoclonal Antibody, Hybridoma, Phage Display, Expression Systemمقالات مرتبط جدید
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