Serum evaluation of enterotoxemia multivalent nanotoxoid vaccine in target animal
- سال انتشار: 1402
- محل انتشار: بیست و چهارمین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM24_343
- زبان مقاله: انگلیسی
- تعداد مشاهده: 103
نویسندگان
چکیده
The role of technology in vaccine development and research is gradually increasing. The use of nanoparticles in vaccine formulations not only increases the stability and immunogenicity of antigens but also targets the arrival of the vaccine to the effect area. Clostridium bacteria are pathogenic due to the secretion of toxins. The purpose of this project is to evaluate the serum of the multivalent nanotoxoid vaccine in the target animal based on British and European pharmacopeias. For this purpose, beta (βNTD-) and epsilon (ɛNTD-) nanotoxins of the vaccinal strains of Clostridium perfringens types B (CN۲۲۸) and (CN۴۰۹) D and alpha nanotoxin (NTD-α) of the vaccinal strain (CN۹۱۳) of Clostridium septicum after confirming the test Quality control methods based on nanoparticle concentration were used to prepare multivalent nanotoxoid vaccine formulation. According to the vaccination program, the candidate vaccine was injected into different groups of sheep. The positive control group received the quadruple clostridial enterotoxemia vaccine from Razi Institute. Indirect ELISA method was used to evaluate the antibody titer. Antibody response measurement showed high stimulation of immunogenicity and stable increase of antibody titer in groups immunized with candidate vaccine, vaccine coated with red cell membrane. The results of the study showed that sheep vaccinated with nanoparticles coated with red cell membranes show higher protective immunity (P-Value < ۰.۰۵). And the red cell membranes provide a safe layer to inhibit the nanotoxin compared to uncoated nanotoxins that cause the slow and gradual release of antigens. By using the membrane of red blood cells, the virulence of the toxin can be neutralized through the spontaneous trapping of particles in these membranes with high efficiency. The reaction of the toxin and the nanoparticle leads to the creation of a new form of nanoparticles that lose their lethality due to the involvement of the toxin in it, and these nanoparticles can easily be separated from the cell membrane and placed in the intercellular spaces due to their new shape. Stimulate the immune system.کلیدواژه ها
Nano toxoid vaccine, Clostridium, Enterotoxemiaمقالات مرتبط جدید
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