Encapsulation of rOmpA from Klebsiella pneumoniae in Silk Fibroin-Sodium Alginate Nanoparticles: A New Approach to Developing a Pneumonia Vaccine
- سال انتشار: 1402
- محل انتشار: بیست و چهارمین کنگره بین المللی میکروب شناسی ایران
- کد COI اختصاصی: MEDISM24_162
- زبان مقاله: انگلیسی
- تعداد مشاهده: 105
نویسندگان
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
چکیده
BACKGROUND AND ABJECTIVEThe present study describes the design and fabrication of a new vaccine candidate based on the outer membrane protein A (rOmpA) from Klebsiella pneumoniae (K. pneumoniae) encapsulated in silk fibroin-sodium alginate nanoparticles (SF-SANPs) against K. pneumoniae -mediated pneumoniae.MATERIALS AND METHODSThe physicochemical properties, toxicity, release profile, and in vivo potency of SF-SANPs encapsulated with rOmpA were evaluated.RESULTS AND DISCUSSIONThe spherical nano vaccine was created with an average particle size of ۱۶۰ nm and an encapsulation efficiency of ۸۰%. Antigen release from SF-SANPs was ۴۰% after ۲۲ days release assay. The SF-SANPs showed a zeta potential of -۲۴.۸mV and had no toxic effect on the L۹۲۹ cells in vitro. The study also found that SF-SANPs in the vaccine formulations promoted systemic (IgA, total IgG, IgG۱ (Th۲), and IgG۲a (Th۱)) and mucosal (IgA and IgG) antibodies and stimulated cytokine response (IFN-γ, IL-۴, and IL-۱۷), inducing both humoral (Th۲) and cell-mediated (Th۱) immune responses, with a Th۱-polarized response.CONCLUSIONThe vaccine was effective in protecting the lung against experimental pneumoniae and reducing inflammation. These findings suggest that the rOmpA-based vaccine encapsulated in SF-SANPs could be a promising strategy for preventing pneumonia caused by K. pneumoniae.کلیدواژه ها
Silk fibroin-sodium alginate nanoparticles, Nanoadjuvant, Vaccine, Pneumoniae, Klebsiella pneumoniaeمقالات مرتبط جدید
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