Silibinin improved the function of T cells in peripheral blood mononuclear cells (PBMCs) co-cultured with U-۸۷ MG cell line
- سال انتشار: 1403
- محل انتشار: مجله گیاهان دارویی ابن سینا، دوره: 14، شماره: 2
- کد COI اختصاصی: JR_AJP-14-2_002
- زبان مقاله: انگلیسی
- تعداد مشاهده: 151
نویسندگان
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran. Brain Cancer Research Core, Universal Scientific Education and Research Network (USERN), Kerman, Iran
Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Department of Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
چکیده
Objective: Silibinin has exhibited antitumor activities. However, there are few reports about the immunomodulatory properties of silibinin on T lymphocyte function in the tumor microenvironment. Here, we determined the effects of silibinin on T cells of peripheral blood mononuclear cells (PBMCs), cultivated alone or with a human cell line of glioblastoma (U-۸۷ MG).Materials and Methods: The proliferation of T lymphocytes was assessed by MTT test in the presence of silibinin (۱۵ and ۴۵ µM). Also, total antioxidant capacity (TAC), the activity of superoxide dismutase-۳ (SOD۳), and the levels of two cytokines interferon gamma (IFN-γ) and tumor growth beta (TGF-β) were compared between treated and untreated PBMCs alone or co-cultured with U-۸۷ cells.Results: According to our results, silibinin raised the TAC levels and SOD۳ activity in the PBMCs and in the co-culture condition. Moreover, silibinin-treated PBMCs showed higher IFN-γ levels and lower TGF-β levels. Interestingly, silibinin protected PBMCs against the U-۸۷-induced suppression.Conclusion: Altogether, these results proposed the immunomodulatory potential of silibinin on T cells of PBMCs, as well as its partially protective effects on PBMCs against the suppression induced by U-۸۷ MG cells.کلیدواژه ها
Silibinin, Glioblastoma, IFN-γ, TGF-β, Peripheral blood mononuclear cells (PBMCs)اطلاعات بیشتر در مورد COI
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