Detection of Metallo-β-Lactamases (MBLs) Producing Pseudomonas aeruginosa Isolates in Tehran Hospitals, Iran

  • سال انتشار: 1396
  • محل انتشار: مجله میکروبیولوژی پزشکی و بیماریهای عفونی، دوره: 5، شماره: 3
  • کد COI اختصاصی: JR_JMMI-5-3_003
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 93
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نویسندگان

Mohsen Tabasi

Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran

Reza Azizian

Massoud Clinical Laboratory, Tehran, Iran

Mohammad Reza Eskandarion

Iranian Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran

Mehri Habibi

Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran

Mohammad Reza Asadi Karam

Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran

چکیده

Introduction: The strains of Pseudomonas aeruginosa are known as an opportunistic pathogen that can cause infections in humans and animals. Metallo-β-lactamases (MBLs) are the most significant factors of resistance to carbapenem antibiotics in these bacteria. This study was designed to identify the MBLs producing P. aeruginosa isolates in three hospitals of Tehran, Iran. Methods: Totally, we obtained ۶۶۵ samples from patients hospitalized in three hospitals in Tehran, Iran. Antibiotic-susceptibility test of the P. aeruginosa isolates was done based on Kirby-Bauer disk diffusion test. The Minimum Inhibitory Concentration (MIC) of the isolates was performed using agar dilution method, and IPM-EDTA test identified MBL producing isolates. Results: Among the examined isolates, ۴۷۳ (۷۱.۱%) were P. aeruginosa. Among these, ۳۰۶ (۶۴.۷%) were resistant to imipenem, and ۲۸۹ (۹۴.۵%) were MBL producers. Furthermore, the resistance rate of the isolates to other antibiotics was amikacin (۲۶%), tobramycin (۲۴.۹۵%), ceftazidime (۲۳.۰۵%), gentamicin (۲۲.۸۳%), carbenicillin (۲۱.۱۴%), and ceftizoxime (۱۸.۱۹%). The MICs of imipenem and ceftazidime for the majority of the isolates were ۴ µg/ml and ˃۱۲۸ µg/ml, respectively. Conclusion: This study confirmed previous reports on the increased rate of MBL-mediated resistance in P. aeruginosa isolates worldwide. Therefore, detection of resistance patterns for these isolates, particularly MBLs, is necessary for prevention and control of Pseudomonas associated infections.

کلیدواژه ها

Pseudomonas aeruginosa, Metallo-β-lactamase, IPM-EDTA, MIC

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