The Effect of Silibinin on the Expression of TLR۷, ISG۱۵, and SOCS۱ in Peripheral Blood Mononuclear Cells of Hepatitis C Infected Patients in Comparison with Interferon-α
- سال انتشار: 1399
- محل انتشار: مجله میکروبیولوژی پزشکی و بیماریهای عفونی، دوره: 9، شماره: 1
- کد COI اختصاصی: JR_JMMI-9-1_003
- زبان مقاله: انگلیسی
- تعداد مشاهده: 41
نویسندگان
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Gastroenterohepatology Research center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Gastroenterohepatology Research center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Introduction: Silibinin (silibinin A) is the most active silymarin component, which acts both as a hepatoprotective [۱] and an antiviral agent. The present study investigated the silibinin effect on IFN-related innate immune genes in PBMCs from HCV-infected patients. Method: ۲۲ chronic HCV patients, including ۱۰ IFN responders and ۱۲ non-responders, were included. Their isolated PBMCs were treated for ۶ hours in the presence of silibinin, IFN-α, or their combination. The transcription level of TLR۷, ISG۱۵, and SOCS۱ genes was compared using real-time PCR. Result: Our result showed that IFN-α induced a significant up-regulation of TLR۷ and ISG۱۵ in PBMCs of both responder and non-responder groups. Nevertheless, the SOCS۱ gene was not significantly changed in the non-responder group (P=۰.۳۲). The combination of IFNα- and silibinin showed a similar pattern to IFN-α alone. By itself, silibinin did not leave a significant change on the expression level of the studied genes. Conclusion: The results indicated that silibinin did not enhance or suppress the expression level of TLR۷, ISG۱۵, and SOCS۱ genes. Therefore, it has been suggested that its anti-inflammatory role might be devoid of IFN pathways.کلیدواژه ها
HCV, Silibinin, Interferon, ISG۱۵, SOCS۱اطلاعات بیشتر در مورد COI
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