Mangiferin and mangiferin-containing leaf extract from Mangifera foetida L for therapeutic attenuation of experimentally induced iron overload in a rat model

  • سال انتشار: 1398
  • محل انتشار: Journal of Herbmed Pharmacology، دوره: 8، شماره: 1
  • کد COI اختصاصی: JR_HERM-8-1_004
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 78
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نویسندگان

Ari Estuningtyas

Tri Wahyuni

Pustika Amalia Wahidiyat

Ernie H Poerwaningsih

Hans-Joachim Freisleben

چکیده

Introduction: Thalassemia is a hereditary anemia usually treated with regular blood transfusions, which can result in elevated levels of total iron in the body. As soon as the capacity of iron-regulating proteins (e.g., ferritin, transferrin) are used up, free plasma iron increases generating reactive oxygen species (ROS), leading to oxidative stress. Hence, blood transfusions should be accompanied by iron-chelating therapy, e.g., deferiprone (DFP). The purpose of this study was to evaluate the effect of mangiferin (M) and an aqueous leaf extract of Mangifera foetida L (EMF) as alternative iron-chelating antioxidants in an animal model.Methods: Thirty male Sprague Dawley rats were randomly divided into ۵ equal groups: normal control, iron overload (IO), IO+DFP, IO+M, and IO+EMF. Iron overload was induced by intraperitoneal iron dextran injection with a total dose of ۹۰ mg/mouse (۱۵ mg Fe/mouse every ۳-۴ days for ۳ weeks) followed by oral administration of DFP ۴۶۲.۵ mg/kg, mangiferin ۷۵ mg/kg, or EMF ۲.۹۳۰ g/kg for ۷ days.Results: Body weight (BW) increased in all groups during the ۴ weeks of experiment, except for the IO group. As expected, DFP decreased significantly the total plasma iron and increased iron excretion via urine in iron-overloaded rats (positive control), mangiferin and EMF had similar – although slightly smaller – effects than DFP. The antioxidant activity of M and EMF were stronger compared to DFP as determined by plasma superoxide dismutase (SOD) activity.Conclusion: Mangiferin and EMF have iron-chelating and antioxidant effects and might be used for the treatment of iron overload in the body.

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