Molecular cloning and in silico analysis of a GTP cyclohydrolase I gene from grape
- سال انتشار: 1402
- محل انتشار: دوفصلنامه اصلاح مولکولی گیاهان، دوره: 11، شماره: 1
- کد COI اختصاصی: JR_JPMB-11-1_001
- زبان مقاله: انگلیسی
- تعداد مشاهده: 61
نویسندگان
Department of Biotechnology Engineering, Faculty of Agricultural and Natural Resources, Imam Khomeini International University, Qazvin, Iran
Department of Biotechnology Engineering, Faculty of Agricultural and Natural Resources, Imam ‎Khomeini International University, Qazvin, Iran‎
Department of Biotechnology Engineering, Faculty of Agricultural and Natural Resources, Imam Khomeini International University, Qazvin, Iran
Department of Biotechnology Engineering, Faculty of Agricultural and Natural Resources, Imam Khomeini International University, Qazvin, Iran
چکیده
An entire open reading frame (ORF) encoding for a polypeptide of GTP cyclohydrolase I (GTPCH I) was isolated and cloned from Askari cultivar of grape (Vitis vinifera L.) berries. The ۱,۳۳۸-nucleotide ORF yields a ۴۴۵-residue amino acid sequence with a calculated molecular mass of ۴۸.۶۵ kDa and a predicted isoelectric point of ۶.۴۳. The Vvgtpch I genomic sequence with a length of ۴,۹۶۴ bp contains two exons (۱۶۹ and ۱,۱۶۹ bp) and an intron (۲,۶۷۶ bp). The gtpch I sequence of grape displayed a strong similarity with gtpch I sequence found in other plants, including peach (۷۲%), cocoa (۷۲%), strawberry (۷۰%), and poplar (۶۹%). Analysis of mRNA secondary structure revealed that the start codon of Vvgtpch I is completely exposed, suggesting a robust binding of the ribosome and efficient translation. Similar to gtpchs I from diverse sources, molecular modeling uncovered that the monomer of VvGTPCH I adopts an αβ structure, which includes ۱۰ α-helices and ۸ β-sheets. Moreover, in silico analysis of the Vvgtpch I gene promoter identified potential cis-acting elements responsive to environmental signals. This suggests that the Vvgtpch I gene has the capacity to be responsive to various environmental cues, such as heat, heavy metals, light, and plant hormones.کلیدواژه ها
Folate, in silico analysis, Regulatory elements, Cloning, Promoter regionاطلاعات بیشتر در مورد COI
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