Molecular study of antibiotic resistance patterns and prevalance of Tem-۱ gene in Klebsiella pneumonia clinicalisolates from patients samples in Ahvaz,Iran

سال انتشار: 1402
محل انتشار: دومین همایش بین المللی زیست شناسی و علوم آزمایشگاهی
کد COI اختصاصی: ZISTCONF02_011
زبان مقاله: انگلیسی
تعداد مشاهده: 64

نویسندگان

Department of Microbiology and Microbial biotechnology , Faculty of life sciences andbiotechnology,Shahid Behishti University, Tehran

Gholam Abbas Kaydani

Assistant Professor of Medical Virology Department of Laboratory Sciences, School of Allied MedicalSciences Ahvaz Jundishapur University of Medical Sciences, Ahvaz.

Neda Soleimani

Department of Microbiology and Microbial biotechnology , Faculty of life sciences andbiotechnology,Shahid Behishti University, Tehran.

چکیده

IntroductionKlebsiella pneumonia is one of the most common ESBL producing micro-organisms.ESBL is a β-lactamase thatcauses resistance to penicillin, first, second and thirdgeneration cephalosporins, and aztreonam by hydrolyzingthese compounds. The TEM gene is one of the ESBL-encoding genes that cause resistance to ampicillin, penicillinand first-generation cephalosporins such as cephalothin. The aim of this study is to determine the prevalence ofESBL producing Klebsiella pneumoniae mainly (TEM-۱ gene) and detected resistance antibiotic pattern isolatedfrom different clinical specimens from different hospitals in Ahvaz,Iran.MethodThis study included ۱۱۰ isolates of K. pneumoniae from various clinical samples which were obtained fromdifferent hospitals in Ahvaz,Iran,during a period from February to May ۲۰۲۳.The isolated organisms wereidentified by using biochemical tests.The sensitivity pattern of these isolates to۱۰ disc Antibiotics was studiedby the Kirby-Bauer disk diffusion Method according to Clinical Laboratory Standards Institute (CLSI) guidelines.PCR was performed for the molecular detection of TEM-۱ gene.Discussion and conclusionIn this study, ۱۱۰ samples of Klebsiell pneumoniae were collected from the diffrent hospitals in Ahvaz.Antimicrobial susceptibility results showed that the highest level resistance of K. pneumoniae was againstceftazidime ۶۶.۲% and lowest level resistance of it was against Amikacin ۱۵.۴%.The investigated isolate’spatterns of antibiotics resistance were as follow:۶۶.۲% resistant for ceftazidime,۶۳% for tetracycline ,۴۱.۱% forCefotaxime,,۳۹.۲% for Cefepime, ۳۹% for gentamicin , ۳۶% for ciprofloxacin , ۳۴.۱% for meropenem , ۳۰.۵% forTrimethoprim/Sulfamethoxazole, ۲۴.۶% for Impenem, ۱۵.۴% for Amikacin . The results of PCR was ۷۳(۶۶.۳%)of Klebsilla pneumonia clinical isolates positive for TEM-۱ gene. results of this study indicate the high frequencyof drug resistance among K. pneumoniae isolated from hospitals of Ahvaz present study shows the presence ofhigh levels of drug-resistant genes in various antibiotics, which are usually used in the treatment of infectionsdue to K. pneumonia.

کلیدواژه ها

Klebsiella pneumoniae, antibiotic resistance, ESBL, TEM-۱ gene

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