All-trans retinoic acid and fibroblast growth factor-۲ enhance the fertility rate and embryo development in polycystic ovary syndrome mouse model
- سال انتشار: 1403
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 27، شماره: 4
- کد COI اختصاصی: JR_IJBMS-27-4_003
- زبان مقاله: انگلیسی
- تعداد مشاهده: 61
نویسندگان
Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Department of Anatomical Sciences, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
Cellular and Molecular Research Center, Research Institute for Non-communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
Cellular and Molecular Research Center, Research Institute for Health Development, Department of Anatomical Sciences, Kurdistan University of Medical Sciences, Sanandaj, Iran
Cellular and Molecular Research Center, Research Institute for Health Development, Department of Anatomical Sciences, Kurdistan University of Medical Sciences, Sanandaj, Iran
Medical Plants Research Center, Basic Health Sciences Institute, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
Department of Epidemiology and Biostatistics, Shahrekord University of Medical Sciences, Shahrekord, Iran
Department of Anatomical Sciences, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
چکیده
Objective(s): Polycystic ovary syndrome (PCOS) causes a developmental arrest of antral follicles and disrupts oocyte maturation. Retinoic acid (RA) and Fibroblast Growth Factor-۲ (FGF۲) are effective in follicle growth, thus their effects on histopathology and in vitro fertility of oocytes were investigated in PCOS-induced mice.Materials and Methods: Eighty female NMRI mice were randomly divided into ۸ groups including ۱-Normal mice, ۲-PCOS mice without any treatment, ۳-Normal mice treated with RA, ۴-Normal mice treated with FGF۲, ۵-PCOS mice treated with RA, ۶- PCOS mice treated with FGF۲, ۷- PCOS mice treated with RA and FGF۲, and ۸- Normal mice treated with RA and FGF۲. Following PCOS induction, the mice were treated with intraperitoneal RA and FGF۲ as a treatment. Then ovarian stimulation, for preparing the oocyte and embryo microscopic examinations was performed. After oocyte morphometry, through in vitro fertilization, the embryo formation was assessed. Data was analyzed by one-way ANOVA and Tukey tests.Results: The results showed simultaneous injection of RA and FGF۲ into PCOS-induced mice increases antral follicles and corpus luteum, but decreases cystic follicles. Simultaneous injection of these two substances into healthy mice increases the pre-antral follicles and corpus luteum. Simultaneous injection of RA and FGF۲ increases the number of embryos in both control and intervention groups. Conclusion: It can be concluded that RA and FGF۲ increase the maturity of ovarian follicles, the number of two-celled embryos, and the number of grade-A embryos in mice with PCOS, which is more effective when these two substances are injected simultaneously.کلیدواژه ها
Embryo development, Fertility agents, Growth Factor, In vitro oocyte maturation - techniques, Reproductionاطلاعات بیشتر در مورد COI
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