Acute and Subchronic Toxicity and Cytotoxicity of Saffron Corm Extract
- سال انتشار: 1403
- محل انتشار: مجله گیاهان دارویی و محصولات فرعی، دوره: 13، شماره: 1
- کد COI اختصاصی: JR_JJMPB-13-1_002
- زبان مقاله: انگلیسی
- تعداد مشاهده: 46
نویسندگان
Agricultural Engineering Research Department, Khorasan Razavi Agricultural and Natural Resources and Education Center, AREEO, Mashhad, Iran
Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
چکیده
Saffron corm is produced annually in large quantities in Iran. It contains some bioactive components like phenolic and antioxidative compounds. We extracted saffron corm by ultrasound-assisted solvent extraction method. The extraction yield and antioxidant properties, free radical scavenging ability, FRAP, and total phenolic compounds (TPC), were determined. To evaluate acute and subchronic toxicity, the extract was administered at three different doses of ۱, ۱۰, and ۱۰۰ mg/kg to BALB/c male mice. Then, the rate of mortality and biochemical parameters such as LDH, ALT, AST, ALP, TG, cholesterol and creatinine were measured after ۲۴ hours and ۶۰ days respectively. Effect of extract on healthy (HFF) and cancerous cells (MCF-۷ & HT-۲۹) was evaluated using MTT assay. Results showed that the ۴۰ min ultrasound-assisted extraction method with the highest extraction yield (۰.۹۸%), free radical scavenging inhibition (۶۴.۳۷%), and highest FRAP (۶۴۳.۵۱ µmolfeII.l-۱) and TPC (۸۲.۲۳ mg.ml-۱) could be selected as the most active saffron corm extract. According to our animal study after acute and subchronic administration of extract, no significant alteration of the levels of TG, cholesterol, ALP, ALT, AST and creatinine was observed, in mice. Cytotoxicity tests showed that saffron corm extract had no toxic effect on HFF cells, although it had toxic effects on MCF-۷ & HT-۲۹ cells. Conclusively, to our data, saffron corm extract had no significant acute and subchronic toxicity on BALB/c mice, and although it had toxic effects on cancer cells no cytotoxicity was seen on normal fibroblast cells.کلیدواژه ها
Saffron corm, ultrasound-assisted extraction, Acute toxicity, Subchronic Toxicity, Cytotoxicityاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.