Study of Nanofibrils Formation of Fibroin Protein in Specific Thermal and Acidity Conditions

  • سال انتشار: 1399
  • محل انتشار: مجله فیزیک و مهندسی پزشکی، دوره: 10، شماره: 1
  • کد COI اختصاصی: JR_JBPE-10-1_005
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 74
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نویسندگان

M Ahrami

MSc, Department of Nanomedicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran

M Khatami

MSc, NanoBioeletrochemistry Research Center, Bam University of Medical Sciences, Bam, Iran

H Heli

PhD, Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

چکیده

Background: Amyloid fibrils are insoluble arranged aggregates of proteins that are fibrillar in structure and related to many diseases (at least ۲۰ types of illnesses) and also create many pathologic conditions. Therefore understanding the circumstance of fibril formation is very important.Objectives: This study aims to work on fibrillar structure formation of fibroin (as a model protein).Material and Methods: In this experimental study, fibroin was extracted from bombyx mori silk cocoon, and the concentration was obtained by Bradford method. The protein was incubated in a wide range of times (۰ min to ۷ days) in specific acidity and thermal conditions (pH=۱.۶, T=۷۰ °C). The assays of UV-vis spectroscopy with congo red, field emission scanning electron microscopy, transmission electron microscopy, atomic force microscopy and circular dichroism spectroscopy were employed to monitor the fibrillation process. Results: Fibroin assemblies were formed upon the process of aggregation and fibril formation with a variety of morphology ranging from nanoparticles to elongated fibrils. Conclusion: The results showed progressive pathway of fibril formation.

کلیدواژه ها

Silk Fibroin, Circular Dichroism, Amyloid Fibrils, Microscopy, Electron, Scanning, Transmission Electron Microscopy, Fibroins

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