An Aptamer-based Biosensor for Troponin I Detection in Diagnosis of Myocardial Infarction
- سال انتشار: 1397
- محل انتشار: مجله فیزیک و مهندسی پزشکی، دوره: 8، شماره: 2
- کد COI اختصاصی: JR_JBPE-8-2_003
- زبان مقاله: انگلیسی
- تعداد مشاهده: 49
نویسندگان
Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI.Objectives: In this study, a molecular method is introduced to early diagnosis of MI by rapid detection of TnI.Materials and Methods: The detection method was based on electrochemical aptasensing, being developed using different methods and evaluation steps. A gold electrode was used as a transducer to successful immobilize ۷۶base aptamer to fabricate a TnI biosensor.Results: The designed aptasensor could detect TnI in a range of ۰.۰۳ to ۲.۰ ng mL-۱ without using any label, pre-concentration or amplification steps. The limit of detection was attained as ۱۰ pg mL-۱ without significant trouble of interfering species. The TnI biosensor demonestrated a stable, regenerative and reproducible function. ۸۹ human samples were used to evaluate the performance of the TnI biosensor, and it represented ۱۰۰% and ۸۱%, diagnostic sensitivity and specificity, respectively.Conclusions: This aptasensor may be used as an applicable tool in the future of early medical diagnosis of MI.کلیدواژه ها
Electrochemical Biosensor, Biomarker, Heart Failure, Troponin, Molecular Detectionاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.