Transcriptional effects of Organochlorine o,p′-DDT and its Metabolite p,p′-DDE in Transfected MDA-MB ۲۳۱ and MCF-۷ Breast Cancer Cell Lines
- سال انتشار: 1394
- محل انتشار: مجله تحقیق در پزشکی مولکولی، دوره: 3، شماره: 2
- کد COI اختصاصی: JR_REMJ-3-2_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 144
نویسندگان
Department of Biology, Faculty of Food Industry and Agriculture, Standard Research Institute, Karaj, Iran
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Department of Quality Control, Razi Vaccine and Serum Research Institute, Karaj, Iran
چکیده
Background: The organochlorine DDT has estrogenic activity but the mechanism underlying the estrogenic activity of this pesticide remains unclear. In the present investigation here, we studied the transcriptional effects of a synthetic organochlorine pesticide o,p’-DDT [۱.۱.۱.-trichloro-۲-(o-chlorophenyl)-۲-p-chloriphenyl ethane] and its metabolite p,p'-DDE (۲-۲-bis(۴/chlorophenyl)-۱-۱-dichloroethyl) on the bovine oxytocin and the thymidine kinase-ERE promoter by estrogen receptor &alpha in MDA-MB ۲۳۱ and MCF-۷ breast cancer cell line. Materials and Methods: Cells were seeded for transfections into ۱۲- well plates at a density of ۱۰۰۰۰۰ cells per well and were transfected with a total of ۳ &mug of plasmid DNA using calcium phosphate coprecipitation. o,p’-DDT and p,p'-DDE were used for stimulation of transfected MDA-MB ۲۳۱ and MCF-۷ breast cancer cell lines. Results: The results showed o,p’-DDT has no agonistic activity in MDA-MB ۲۳۱ cells transfected with the oxytocin promoter construct (OTwt) or the thymidine kinase-ERE promoter construct (TK ERE) and estrogen receptor &alpha. While, The results obviously show that o,p’-DDT has an agonistic effect on both the oxytocin and the thymidine kinase-ERE promoter in MCF-۷ leading to a more than two-fold stimulation of transcription at ۱۰-۵ M. In addition, there is no agonistic effect with p,p'-DDE on transfected MCF-۷ cells and MDA-MB ۲۳۱ cell line. Conclusion: In conclusion, our results revealed that o,p’-DDT has not estrogenic activity in a classical mechanism in transfected MDA-MB ۲۳۱ breast cancer cells while has estrogenic activity in a classical mechanism in transfected MCF-۷ human breast cancer cell line.کلیدواژه ها
Organochlorinated pesticides, o, p’-DDT, MCF-۷ cell line, thymidine kinase-ERE promoter.اطلاعات بیشتر در مورد COI
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