Galectin-۹ Expression Profile in Patients with Gastric Cancer and Peptic Ulcer Disease
- سال انتشار: 1395
- محل انتشار: مجله تحقیق در پزشکی مولکولی، دوره: 4، شماره: 4
- کد COI اختصاصی: JR_REMJ-4-4_001
- زبان مقاله: انگلیسی
- تعداد مشاهده: 168
نویسندگان
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Department of Internal Medicine, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, Iran
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
چکیده
Background: Chronic inflammation and dys-regulation of the immune system mechanisms are now well established as primary triggers of gastric cancer and peptic ulcer disease. Galectin-۹ (Gal-۹) is a member of the galectin family and known as an inducer of cell aggregation, adhesion and apoptosis. Gal-۹ interaction with its main ligand T-cell immunoglobulin and mucin domain protein-۳ (Tim-۳) leads to the apoptosis of T cells and dys-regulation of the immune responses in different tumors. In the current study, the mRNA expression pattern of Gal-۹ was evaluated in gastric biopsies of patients with gastric cancer and peptic ulcer disease. Methods: In this case control study, gastric biopsies were obtained from ۴۶ patients with gastric cancer, ۴۴ patients with peptic ulcer disease and ۴۱ cases with non-ulcer dyspepsia served as controls who underwent endoscopy for evaluation of their gastric problems. Infection with Helicobacter pylori was determined by rapid urease test for all participants and H&E staining for GC patients. Total RNA was extracted from all gastric tissues and used for cDNA synthesis. Relative expression of Gal-۹ mRNA was determined by Real-Time PCR using β-actin as a housekeeping gene. Results: Gal-۹ was similarly expressed in all three studied groups. No statistical difference was found for Gal-۹ expression between gastric cancer patients and control group (۲-ΔCt =۰.۰۲۲ vs ۰.۰۱۴۴, p=۰.۳۰) and also between peptic ulcer and control groups (۲-ΔCt =۰.۰۸۸ vs ۰.۱۴۴, p=۰.۱۶). No correlation was found for Gal-۹ expression and infection with Helicobacter pylori (۲-ΔCt = ۰.۱ vs ۰.۱۲۹, p=۰.۵۱). Conclusion: Similar expression of Gal-۹ in gastric tissues from patients with gastric cancer, peptic ulcer and also non-ulcer dyspepsia individuals suggest no possible role of this molecule on tumorigenesis and immunoregulatory mechanisms of these gastric disorders.کلیدواژه ها
Gastric cancer, Peptic ulcer disease, Galectin-۹, Helicobacter pyloriاطلاعات بیشتر در مورد COI
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