Comparison of Multiplex Quantitative Real-Time PCR and Culture Methods for the Diagnosis of Bacterial Meningitis in Patients with Suspected Meningitis

  • سال انتشار: 1402
  • محل انتشار: فصلنامه عفونت، اپیدمیولوژی و پزشکی، دوره: 9، شماره: 3
  • کد COI اختصاصی: JR_IEM-9-3_007
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 180
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نویسندگان

Shokouh Ghafari

Cellular and Molecular Research Center, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran. Department of Microbiology and Immunology, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Kokab Namakin

Cardiovascular Diseases Research Center, Department of Pediatrics, Birjand University of Medical Sciences, Birjand, Iran Birjand University of Medical Sciences, Birjand, Iran

Ali Reza khooban

Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran

parvin askari

Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran

Masoud Yousefi

Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran

Masood Ziaee

Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran

چکیده

Background: This study aimed to compare the diagnostic efficacy of standard culture method with multiplex quantitative real-time polymerase chain reaction (qPCR) in examining cerebrospinal fluid (CSF) samples collected from patients with suspected meningitis. Materials & Methods: A retrospective evaluation was conducted on ۱۶۶ patients with suspected meningitis, who were treated in Vali-Asr hospital in Birjand, Iran between ۲۰۱۱ and ۲۰۲۰. Diagnosis of bacterial meningitis was based on CSF culture and multiplex qPCR results. Findings: Among ۱۶۶ patients, conventional methods identified causative pathogens in only ۱۰.۳% of cases, while multiplex qPCR detected pathogens in eight out of ۲۵ culture-negative cases as well. The most common pathogens identified were enterovirus, Epstein-Barr virus, herpes simplex, Haemophilus influenzae, and Streptococcus pneumoniae. Conclusion: Multiplex qPCR appears to be a more effective method than conventional culture in identifying bacterial and viral pathogens that most commonly cause meningitis. The incorporation of qPCR as a routine diagnostic method for meningitis in clinical practice could significantly enhance clinical decision-making and patient care.

کلیدواژه ها

Bacterial meningitis, Culture, Real time polymerase chain reaction

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