Response Surface Methodology for Optimization of Supercritical Fluid Extraction of Orange Peel Essential Oil
- سال انتشار: 1399
- محل انتشار: مجله تحقیقات دارویی و بیومدیک، دوره: 6، شماره: 4
- کد COI اختصاصی: JR_PBRE-6-4_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 67
نویسندگان
Active Pharmaceutical Research Center (APIRC), Pharmaceutical Science Branch, Islamic Azad University, Tehran, Iran.
Department of Chemistry, Faculty of Science, Shahid Beheshti University, Tehran, Iran.
Active Pharmaceutical Research Center (APIRC), Pharmaceutical Science Branch, Islamic Azad University, Tehran, Iran.
Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
چکیده
Background: Orange peel essential oils were obtained using supercritical fluid extraction. This method is an important high scaling extraction method used for the extraction of plant and animal extracts. Objectives: The aim of this study was the optimization of the extraction of the orange peel essential oil. Methods: The experimental parameters of Supercritical Fluid Extraction (SFE) such as temperature, pressure, and extraction time, and modifier volume were optimized using a central composite design after a ۲۴-۱ fractional factorial design. Orange peels were collected from ripe orange and were washed, air dried, and milled. Then, a Suprex MPS/۲۲۵ system in the SFE mode with a maximal operating pressure of ۳۹۵ bars was used for essential oil extraction. Moreover, GC-MS and GC-FID were used for the identification and determination of oil compounds, respectively. Results: Eight compounds have been identified based on their retention indices and mass spectra. According to the results, α-pinene, β-pinene, myrcene, d-limonene, terpinolene, C۸-aldehyde, citronellol, and linalool were identified in orange peel essential oil. The optimum SFE conditions were obtained at a pressure of ۳۴۷.۰۷ atm, temperature of ۵۵° C, extraction time of ۳۰.۱۶ min, and ethanol volume of ۱۴۷.۰۵ µL. Moreover, extraction yields based on SFE varied in the range of ۰.۰۴% to ۱.۱۸% (w/w). Conclusion: The Results showed supercritical fluid technology as the best alternative technique for the extraction of pure and high-quality essential oil from orange peel. It is a green method and does not have any environmental impact.کلیدواژه ها
Essential oils, Extraction, Supercritical fluid extraction, Volatile compoundsاطلاعات بیشتر در مورد COI
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