S.A. Pourbakhsh
H. Goudarzi
M.R. Seyfi Abad Shapouri
H. Keyvanfar
M. Lotfi
M. Kamalzade

With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria ۷۵/۱ strain (۱۶۳۷ bp) was amplified using RT-PCR and purified. It was then cloned into pPICZαA a secretory expression vector of P. pastoris for first time. The insertion was proved by both production of a ۲۱۸ bp segment in Nested PCR and isolation of gene from construct by restriction enzyme (XbaΙ). Finally, It was sequenced. In conclusion, after the expression of fusion (F) gene in p. pastoris expression system, it can be used in production of recombinant vaccine against PPR disease.

Peste des petits ruminants virus (PPRV), Fusion protein, Cloning, P. pastoris, Yeast expression vector