K Fekri - Department of Paramedicine, Amol School of Paramedical Sciences, Mazandaran University of Medical Sciences, Sari, Iran
M. Sh Mortezaie - Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
N Maleki-Dizaji - Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
F Fathiazad - Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
S Hamedeyazdan - Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

The genus Marrubium, belonging to the family Lamiaceae, is highly praised in herbal medicine of different countries for having renowned healing properties. Herein, the anti-inflammatory and anti-angiogenesis potential of Marrubium persicum methanol extract was evaluated in a mouse air pouch model of inflammation. Aerial parts of M. persicum were solvent extracted using the Soxhlet apparatus. Subsequently, air injections were performed (for ۳ days) into the mice's backs to bring about an air pouch, while carrageenan was used to induce inflammation. The mice were divided into four groups, including; negative control (normal saline into the pouch), control (carrageenan), treatment and positive control (dexamethasone). The inflammatory markers were analyzed ۴۸h after injecting carrageenan, and a haemoglobin assay kit assessed the quantification of angiogenesis in granulation tissue. M. persicum methanol extract at doses of ۳.۵, ۵, ۷.۵ and ۱۰ mg/kg represented significant decreases in inflammatory parameters. Compared to the control group, the optimum dose (۳.۵ mg/kg) lessened the myeloperoxidase (MPO) and angiogenesis activity, as well as haemoglobin levels. In sum, the methanol extract of M. persicum exhibited anti-inflammatory effects against carrageenan-induced inflammation, which could be related to its antioxidant and inhibitory effects on neutrophils’ infiltration.

Lamiaceae, methanol extract, Myeloperoxidase, Haemoglobin, neutrophils' infiltration