Comparative Evaluation of Specific Antibody against Mycobacterium tuberculosis ESAT-۶ Recombinant Antigen in Healthy Subject with Positive and Negative Skin Test

  • سال انتشار: 1402
  • محل انتشار: مجله آرشیو رازی، دوره: 78، شماره: 3
  • کد COI اختصاصی: JR_ARCHRAZI-78-3_004
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 122
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نویسندگان

S Ahmadi

Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

N Mosavari

Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

M Tebianian

Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

چکیده

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (M. tuberculosis). The laboratory diagnosis of the disease includes various bacteriologic and immunologic methods. Despite the effectiveness of many of these methods in diagnosing active TB, their high cost and time-consuming nature have led researchers to adopt more accurate and rapid screening methods based on specific antigens for M. tuberculosis. The present study aimed to measure specific antibody serum levels against the early secretory antigenic target ۶ kDa (ESAT-۶) recombinant protein in healthy people and compare it to TB patients. The target population included ۲۷ TB patients and ۸۷ healthy individuals with no clinical TB symptoms. The healthy population was divided into two groups, including positive purified protein derivative (PPD) and negative PPD (۳۵ and ۵۲ people, respectively), using the Tuberculin skin test. The specific antibody level against the ESAT-۶ recombinant antigen and the PPD protein was measured using an indirect Enzyme-Linked Immunosorbent Assay (ELISA) test. The results of the study showed that the majority of the healthy population with no symptoms of clinical TB and having negative skin test results did not have antibodies against the recombinant ESAT-۶ (۹۸%) and PPD (۹۶%) antigens. On the other hand, there was a high level of the specific antibody of the ESAT-۶ recombinant and PPD antigens in TB patients (۷۷%). It is notable that in people with positive skin test results, the level of the antibody against the ESAT-۶ recombinant antigen and PPD antigen was ۹۴%. The results demonstrated that the ELISA method based on the measurement of antibodies against the ESAT-۶ recombinant antigen can be a proper diagnostic method for rapid and accurate screening of healthy from infected people.

کلیدواژه ها

Antibody, ELISA, ESAT-۶, Mycobacterium tuberculosis

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