Does Gold-Silver Core-Shell Nanostructure with Alginate Coating Induce Apoptosis in Human Lymphoblastic Tumoral (Jurkat) Cell Line?
- سال انتشار: 1402
- محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 12، شماره: 2
- کد COI اختصاصی: JR_RBMB-12-2_003
- زبان مقاله: انگلیسی
- تعداد مشاهده: 26
نویسندگان
Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Reproductive Sciences and Technology Research Center, Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran.
Endometriosis Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Endometriosis Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran.
School of Dentistry, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Reproductive Sciences and Technology Research Center, Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran.
چکیده
Background: T-cell acute lymphoblastic leukemia (T-ALL) is known as an aggressive malignant disease resulting from the neoplastic alteration of T precursor cells. Although treatment with stringent chemotherapy regimens has achieved an ۸۰% cure rate in children, it has been associated with lower success rates in adult treatment. Silver nanoparticles (Ag-NPs) have a toxic effect on human breast cancer cells, human glioblastoma U۲۵۱ cells, and chronic myeloid leukemia cells in vitro. This study aimed to investigate the effect of Ag nanostructures (Ag-NSs) on Jurkat cells’ viability and apoptosis. Methods: The Jurkat cell line was acquired. Following the synthesis Ag-NSs and their characterization, they were incubated with Jurkat cells at different doses for ۲۴, ۴۸, and ۷۲ hours to determine the optimal time and dose. Two groups were examined: a control group with Jurkat cells without nanostructure maintained in the same medium as the cells in the treatment group without changing the medium, and a treatment group with cells treated with the Ag nanostructure solution at a dose of ۷۵ µg/ml for ۴۸ hours according to the MTT results. After ۴۸ hours, the cells from the two groups were used for the q RT-PCR of the apoptotic genes (BAX, BCL-۲, and CASPASE-۳). Results: According to our results, the rod-shaped silver nanostructures had a size of about ۵۰ nm, increased apoptotic markers, including BAX and CASPASE-۳, and induced cell death. Conclusions: Ag-NSs have anticancer properties and can induce apoptosis of cells; therefore, they may be a potential candidate for the treatment of T-cell acute lymphoblastic leukemia. Background: T-cell acute lymphoblastic leukemia (T-ALL) is known as an aggressive malignant disease resulting from the neoplastic alteration of T precursor cells. Although treatment with stringent chemotherapy regimens has achieved an ۸۰% cure rate in children, it has been associated with lower success rates in adult treatment. Silver nanoparticles (Ag-NPs) have a toxic effect on human breast cancer cells, human glioblastoma U۲۵۱ cells, and chronic myeloid leukemia cells in vitro. This study aimed to investigate the effect of Ag nanostructures (Ag-NSs) on Jurkat cells’ viability and apoptosis. Methods: The Jurkat cell line was acquired. Following the synthesis Ag-NSs and their characterization, they were incubated with Jurkat cells at different doses for ۲۴, ۴۸, and ۷۲ hours to determine the optimal time and dose. Two groups were examined: a control group with Jurkat cells without nanostructure maintained in the same medium as the cells in the treatment group without changing the medium, and a treatment group with cells treated with the Ag nanostructure solution at a dose of ۷۵ µg/ml for ۴۸ hours according to the MTT results. After ۴۸ hours, the cells from the two groups were used for the q RT-PCR of the apoptotic genes (BAX, BCL-۲, and CASPASE-۳). Results: According to our results, the rod-shaped silver nanostructures had a size of about ۵۰ nm, increased apoptotic markers, including BAX and CASPASE-۳, and induced cell death. Conclusions: Ag-NSs have anticancer properties and can induce apoptosis of cells; therefore, they may be a potential candidate for the treatment of T-cell acute lymphoblastic leukemia.کلیدواژه ها
Apoptosis, Cell viability, Nanostructures, T-cell acute lymphoblastic leukemia.اطلاعات بیشتر در مورد COI
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