Study of the therapeutic effect of fullerene nanoemulsion on wrinkle repair of C۵۷BL۶ mouse animal model with high frequency imaging

Introduction: One of the most obvious morphological changes due to aging is the formation of facial wrinkles, which are remarkably visible at middle ages and can have a significant effect on the quality of life of individuals. It is believed that UVB is one of the main causes of aging by stimulating reactive oxygen species. Nanoemulsion was loaded with fullerene, a strong free radical scavenger, and its efficacy to provide protection and regenerative effect against ROS-induced collagen breakdown in C۵۷BL۶ mouse animal model of skin was studied by recording high-frequency ultrasound images and evaluating the depth of heath layer of the tissue including the dermal and epidermal layer. Materials and Methods: Nanoemulsions formation was carried out using a method based on high energy emulsification technique. Oil phase (Fullerene (C۶۰), white beeswax, Span ۸۰ Phenonip, Palmkerneloilesters) and aqueous phase (xanthan gum, Tween ۸۰, Water deionized) were separately preheated up to ۷۵ ± ۱ C. Fullerene nanoemulsion was prepared by adding the aqueous phase dropwise into the oil phase and homogenizing the mixture at ۳۰۰۰ rpm using a high shear homogenizer at room temperature (۲۵ ± ۲ C) for ۲۰ min. The premixed emulsions were further subjected to ultrasonication by ۲۴ kHz ultrasonic tip processor with a maximum power output of ۴۰۰ W. The C۵۷BL۶ mouse animal model was exposed to radiation induced aging for۵ weeks (۵ times a week) with radiation intensity of ۰/۰۳ mW/ cm۲ and then devided into two groups: control(۵mice) and treatment(۵mice) Subjects were instructed to apply ۰.۵ mL of fullerene nanoemulsion at a concentration of ۱۰۰۰µg/ml for۲۸ days (twice a day in the morning and at nigh) in the form of topical UV radiation. Physical parameters were measured weekly (up to ۴weeks) during the treatment wrinkles from ultrasound images with a resolution of ۰/۰۰۱mm and in the groups analyzed as mean and standard deviation. Results: The percentage of changes in the thickness of the epidermis layer on the seventh day in the control group increased by ۲%, in the treatment group with a fullerene nanoemulsion decreased by۱۳%, on the ۱۴th day in the control group increased by ۵%, and in the treatment group decreased by ۳۲%, on the ۲۱th day in the control group and treatment decreased by ۰% and ۳۷% and on the ۲۸th day in the control group and treatment groups, respectively increased by ۲% and decreased by ۴۳%. The percentage of changes in the thickness of the dermis layer on the seventh day in the control group increased by ۱%, in the treatment group with a fullerene nanoemulsion decreased by۲۱%, on the ۱۴th day in the control group increased by ۴%, and in the treatment group decreased by ۲۸%, on the ۲۱th day in the control group and treatment decreased by ۳% and ۳۴% and on the ۲۸th day in the control group and treatment groups, respectively increased by ۱% and decreased by ۳۷%. Conclusion: The physicsl parameters of the skin layers resulting from the processing of ultrasound images (thickness or skin layers) significantly decreased during the treatment process in the animal model of the mouse. Therefore in the present study the effect of the fullerene nanoemulsion regeneration on the basis of the processing of consecutive ultrasound images with high resolution in wrinkle repair is proposed.

کلیدواژه ها:

wrinkles ، Fullerene nanoemulsion high-frequency ultrasound