Gold nanoparticles can induce more apoptosis and double strand breaks on HT-۲۹ cells irradiated by ۱۸ MV photons
- سال انتشار: 1397
- محل انتشار: مجله فیزیک پزشکی ایران، دوره: 15، شماره: 0
- کد COI اختصاصی: JR_IJMP-15-0_305
- زبان مقاله: انگلیسی
- تعداد مشاهده: 159
نویسندگان
Nanotechnology Research Center, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Associate Professor, Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz,
Assistant Professor, Department of Virology, School of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran.
Associate Professor, Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Associate Professor, Department of Medicinal Chemistry, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Radiology and Nuclear Medicine, School of Para Medical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran.
چکیده
Introduction: To increase therapeutic ratio, using radio-sensitizers is recommended and nowadays some types of them are utilized clinically. In recent years, Gold nanoparticles (GNPs) were considered as radio-sensitizers in many studies due to high atomic number. Although theoretical works showed that GNPs have an insignificant effect at MV energies, some In-vitro and In-vivo studies have proven promising result for using GNPs at the energy range. The work aims to investigate the effect of GNPs on apoptosis induction and double strand breaks (DSBs) incidence for colon cancer cell line (HT-۲۹) at ۱۸ MV energy. Materials and Methods: The GNPs were synthesized by seeding-growth method. To determine the morphology and size distribution of the synthesized GNPs, transmission electron microscopy (TEM) was used. The cytotoxicity of GNPs on HT-۲۹ cells was assessed by MTT method in various concentration of GNPs. Moreover, the uptake extent of the nanoparticles into the cells was measured by graphite furnace atomic absorption spectroscopy (GF-AAS). The cells irradiation was done by ۱۸ MV photons after incubation with GNPs. For apoptosis test, ۲۴ h after irradiation, the cells were prepared and read by flowcytometer. As a well-known biomarker of DSBs in cells, γ-H۲Ax foci formation were investigated by flowcytometer. Results: The mean size of synthesized GNPs was ۲۴.۷۵ nm and standard deviation ۳.۶ nm. TEM images showed spherical like shape for the GNPs. The cytotoxicity of GNPs on Ht-۲۹ cells showed a time-dependent manner so that it was lower for incubation time ۴۸ h than ۲۴ h. Additionally, by increase in GNPs concentration the viability rate of the cells was reduced. The uptake results also exhibited a time dependent pattern and it reached to the maximum after ۱۲ h of incubation time. Apoptosis test showed that the GNPs treated group has significantly more apoptotic cells than other group (P< ۰.۰۵). Moreover, GNPs caused to forming more γ-H۲Ax foci in ۱۸ MV irradiated HT-۲۹ cells. Conclusion: Considering result of apoptosis and γ-H۲Ax foci formation tests, it seems the GNPs have a great potential to utilize as radio-sensitizer for colon cancer cells at ۱۸ MV energy. However, it needs to study the effect under various conditions as well on animal models.کلیدواژه ها
Gold Nanoparticles, Radiotherapy, Double Strand Break, Apoptosis, Radio-sensitivityاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.