Identification of bioactive constituents of chloroform fraction from Annona muricata leaf, its antioxidant activity and inhibitory potential against carbohydrate-hydrolyzing α-amylase and α-glucosidase activities linked to type II diabetes mellitus: In vitro study

  • سال انتشار: 1401
  • محل انتشار: Journal of Herbmed Pharmacology، دوره: 12، شماره: 1
  • کد COI اختصاصی: JR_HERM-12-1_009
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 172
دانلود فایل این مقاله

نویسندگان

Oluwaseun Ruth Olasehinde

Department of Chemical Sciences, Phytomedicine and Nutraceutics Unit, Biochemistry Programme, Afe Babalola University, P.M.B ۵۴۵۴, Ado-Ekiti, Ekiti State, Nigeria

Olakunle Bamikole Afoladi

Department of Chemical Sciences, Phytomedicine and Nutraceutics Unit, Biochemistry Programme, Afe Babalola University, P.M.B ۵۴۵۴, Ado-Ekiti, Ekiti State, Nigeria

چکیده

Introduction: Secondary metabolites from plants have been found to play an important role in the treatment of diabetes mellitus (DM) and its complications. Therefore, the purpose of this study was to identify the chemical components of Annona muricata leaf chloroform fraction (CFAm) and its in vitro antioxidant properties, as well as inhibitory activity against α-amylase and α-glucosidase enzymatic activities. Methods: Gas chromatography–mass spectrometry (GC-MS) technique was engaged in the identification of phytochemical constituents. Antioxidant activities such as DPPH free radical scavenging ability, reducing power capacity, hydroxyl radical scavenging ability, singlet oxygen scavenging capacity, as well as α-amylase and α-glucosidase inhibition were carried out using standard in vitro methods. Results: GC-MS analysis of CFAm revealed the presence of ۲۳ phytochemicals, out of which ۵ compounds had the highest % compositions (i.e., octadecanoic acid (۲۰.۳۵%), ۲-propanone, ۱-(۴-hydroxy-۳-methoxyphenyl) (۱۲.۰۴%), isocomene (۲۲.۶۰%), ۹, ۱۲, ۱۵ octadecatrienoic acid, methyl ester (Z,Z,Z) (۲۸. ۹۸%), and quercetin, ۵TMS derivative (۱۸.۲۸%)). Also, CFAm demonstrated a significant (P < ۰.۰۵) inhibition against DPPH (IC۵۰= ۲۶.۳۳ ± ۱.۳۹ mg/mL), with OH free radical scavenging capacity (۶۵.۴۶ ± ۱.۳۹ mg/۱۰۰ g), singlet oxygen scavenging capacity (۵۵.۲۴ ± ۱.۲۲ mg/۱۰۰ g), and showed ferric reducing power (۸۴.۵۲ ± ۲.۸۴ mg/۱۰۰ g). Also, CFAm exhibited a significant (P < ۰.۰۵) inhibition against α-glucosidase (IC۵۰= ۷۱.۰۶ ±۱.۴۵ mg/mL) and α-amylase (IC۵۰= ۷۳.۸۸ ± ۱.۵۸ mg/mL) in a concentration-dependent manners. Conclusion: The remarkable properties demonstrated by CFAm, which are essential for the management of DM, could probably be credited to the presence of the various identified phytonutrients.

کلیدواژه ها

Annona muricata, Antioxidant, Antidiabetic, Reducing power, Free radicals

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.