Identification of clinical dermatophyte isolates obtained from Iran by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
- سال انتشار: 1398
- محل انتشار: مجله قارچ شناسی پزشکی، دوره: 5، شماره: 2
- کد COI اختصاصی: JR_CUMM-5-2_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 173
نویسندگان
Mazandaran University of Medical Sciences
Shahid Beheshti University of Medical Sciences, Tehran, Iran
Bushehr University of Medical Sciences, Bushehr, Iran.
Babol University of Medical Sciences, Babol, Iran
Mazandaran University of Medical Sciences, Sari, Iran
Mazandaran University of Medical Sciences, Sari, Iran
Faculty of Medicine, University of Akdeniz, Antalya, Turkey.
Faculty of Medicine, University of Akdeniz, Antalya, Turkey.
aculty of Medicine, University of Akdeniz, Antalya, Turkey.
Department of Microbiology, Faculty of Medicine, University of Çukurova, Adana, Turkey
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
چکیده
Background and Purpose: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is widely used to discriminate among pathogenic microorganisms in clinical laboratories. The aim of this study was to assess the utility of MALDI-TOF MS in the routine identification of clinical dermatophyte isolates obtained from various geographical regions of Iran. Materials and Methods: A total of ۹۴ isolates, including Trichophyton interdigitale (n=۴۴), T. rubrum (n=۴۰), T. tonsurans (n=۴), Microsporum canis (n=۴), and Epidermophyton floccosum (n=۱), were analyzed in this study. The identity of each isolate was determined by polymerase chani reaction amplification and sequencing of the internal transcribed spacer (ITS) region of nuclear-encoded ribosomal DNA and also MALDI-TOF MS. The obtained data by molecular approach were compared with MALDI-TOF MS. Results: The MALDI-TOF MS led to the identification of ۴۴ (۴۷%) isolates at the species level by generating the spectral score values of ≥ ۲.۰. However, there was not sufficient agreement between the results of the molecular-based ITS identification methods and MALDI-TOF MS in the species identification of ۱۶ (۱۷%) isolates. The Bruker Daltonics database was also not able to identify protein spectra related to ۱۲ isolates (۱۳%), including T. interdigitale (n=۵), T. rubrum (n=۴), M. canis (n=۲), and T. tonsurans (n=۱). Conclusion: According to the results, the utility of MALDI-TOF MS as a routine diagnostic tool for the accurate and reliable identification of dermatophytes can be justified whenever the protein spectra of a large set of worldwide clinical isolates are included in the commercial libraries. In addition, MALDI-TOF MS can be alternatively used to construct an in-house reference database.کلیدواژه ها
dermatophytes, Iran, ITS phylogeny, MALDI-TOF MSاطلاعات بیشتر در مورد COI
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