Vitis Elegan as a Promising Antidiabetic Herb: Phytochemical and Pharmacological Assessment
- سال انتشار: 1397
- محل انتشار: مجله گیاهان دارویی و محصولات فرعی، دوره: 7، شماره: 1
- کد COI اختصاصی: JR_JJMPB-7-1_009
- زبان مقاله: انگلیسی
- تعداد مشاهده: 87
نویسندگان
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
Research Center for Molecular and Cellular Imaging, Tehran University of Medical sciences, Tehran, Iran
Cancer Biology Research Center, Cancer Institute of Iran, Tehran University of Medical Science, Tehran, Iran
Department of Surgical Oncology, Cancer Institute of Iran, Tehran University of Medical Science, Tehran, Iran
Department of Genetic, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Young Researchers and Elite Club, East Tehran Branch, Islamic Azad University, Tehran, Iran
چکیده
In this research, we investigated the antidiabetic activity of Vitis elegans rhizome, which is used as traditional treatment for diabetes mellitus. The methanol, chloroform, petroleum ether, and hexane extracts of the plant root were obtained through serial exhaustive extraction and were analyzed by Thin Layer Chromatography (TLC). Glycogen phosphorylase (GP) assay was done to determine the inhibitory effects of respective extracts on GP enzyme. Total phenol content was measured using the Folin-Ciocalteu method and brine shrimp test was done to evaluate the toxicity of the extracts. Evaluation of TLC plates alone and after spraying with different reagents indicated that terpenoid was the major component of the sample followed by alkaloid and phenol. Chloroform extract applied more inhibitory effects on GP enzyme activity with percentages of ۳۹.۶۵ in concentration of ۲.۵ mg/ml. This suppression effect was higher than glucose, as a standard inhibitory agent in the body. The highest amount of phenol was found in the methanol extract, equal to ۴۹ mg GAE g-۱. Petroleum ether, chloroform and methanol extracts were considered as non-toxic solvents with LC۵۰ values of ۸.۹, ۳.۵ and ۳.۷ mg/ml respectively. While hexane with ۰.۰۸۹ mg/ml LC۵۰ value was classified as toxic extract. Based on the results of this study, we concluded that Vitis elegans rhizome, has the potential to be further studied for anti-hyperglycemic properties.کلیدواژه ها
Anti-diabetic, Enzyme Inhibition, Glycogen phosphorylase, Phytochemical screening, Vitis elegansاطلاعات بیشتر در مورد COI
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