Karyological and Nuclear DNA Content Variation in Some Iranian Endemic Thymus Species (Lamiaceae)
محل انتشار: مجله علوم و فناوری کشاورزی، دوره: 12، شماره: 4
سال انتشار: 1389
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 92
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شناسه ملی سند علمی:
JR_JASTMO-12-4_007
تاریخ نمایه سازی: 1 آذر 1402
چکیده مقاله:
Thymus is a medicinal plant which contains one of the world's top ten essential oils, exhibiting antibacterial, antioxidative, food preservative and mammalian age-delaying properties. This work was aimed at identifying between-species variations requiring for selecting appropriate parents for hybridization. Six Iranian endemic Thymus accessions belonging to Thymus daënensis, T. eriocalyx and T. migricus were studied. Root tips were examined for karyological studies and fresh young leaves of the standard reference (Parsley, Petroselinum crispum, ۲C DNA= ۴.۴۵ pg) and the Thymus samples stained with propidium iodide (PI) for flow cytometric (FCM) measurements. Two ploidy levels (diploid and tetraploid) and ۳ chromosome numbers (۳۰, ۵۶, ۶۰) were recognized. The latter chromosome number is being reported for the first time on T. daënensis accession. FCM measurements showed that ۲C DNA contents varied from ۱.۰۲ to ۲.۴۲ pg, verifying more than ۲-fold variations and showing a genome size range of ۴۹۹ to ۱۱۸۲ Mbp, correspondingly. The mean amount of ۲C DNA/chromosome and mean of monoploid genome size were not proportional to ploidy. ۲C-values were correlated with, and linearly regressed upon somatic metaphase, considering either total chromosome volume (TCV) or total chromatin length (X).
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نویسندگان
S. Mahdavi
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, P. O. Box: ۱۴۱۱۵-۳۳۶, Tehran, Islamic Republic of Iran.
G. Karimzadeh
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, P. O. Box: ۱۴۱۱۵-۳۳۶, Tehran, Islamic Republic of Iran.