Investigation of Plant Growth Regulators Effects on Callus Induction and Shoot Regeneration of Bunium persicum (Boiss.) B. Fedtsch.
- سال انتشار: 1388
- محل انتشار: مجله علوم و فناوری کشاورزی، دوره: 11، شماره: 4
- کد COI اختصاصی: JR_JASTMO-11-4_011
- زبان مقاله: انگلیسی
- تعداد مشاهده: 81
نویسندگان
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Mazandaran University, Mazandaran, Islamic Republic of Iran
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Mazandaran University, Mazandaran, Islamic Republic of Iran
چکیده
Parsi Zira, Bunium persicum (Boiss.) B. Fedtsch., or Black Zira, a member of the Umbellifera family, is one of the most important medicinal species with high economic value. Generally, there is limited information about in vitro culture of Bunium persicum. In this study, the effect of various media (MS, B۵) and combinations of plant growth regulators, NAA and ۲,۴-D alone or together with Kin, on callus induction and shoot regeneration from hypocotyl explant were investigated. This experiment has been carried out in a completely randomized design with ۳۰ treatments and ۱۰ replications per treatment. The results showed significant effects of treatments on regeneration and callus induction. All the measured traits for MS medium were superior. The highest callus frequency was observed on the medium containing ۰.۱ mg L-۱ or ۱ mg L-۱ ۲,۴-dichlorophenoxyacetic acid (۲,۴-D) as well as ۲ mg L-۱ ۲,۴-D and ۰.۵ mg L-۱ kinetin. The best treatment for somatic embryogenesis was the medium containing ۲ mg L-۱ ۲,۴-D. The best response for shoot regeneration was observed on the medium supplemented with ۱ mg L-۱ ۲,۴-D. The medium containing ۱ mg L-۱ ۲,۴-D was the best for callus induction and shoot regeneration simultaneously. The regenerated shoots were rooted on basal medium.کلیدواژه ها
Bunium persicum (Boiss.) B. Fedtsch, Hypocotyls explants, Shoot regeneration, Tissue cultureاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.