Development, Characterization, and Influence of Proanthocyanidin Loaded PLGA Nanoparticles on Push out Bond Strength of Glass Fibre Posts Bonded with Resin Cement to Root Dentin: In Vitro Study
- سال انتشار: 1403
- محل انتشار: مجله علوم دارویی و شیمی، دوره: 7، شماره: 1
- کد COI اختصاصی: JR_JMCH-7-1_018
- زبان مقاله: انگلیسی
- تعداد مشاهده: 92
نویسندگان
Department of Conservative Dentistry and Endodontics, Manipal College of Dental Sciences, Manipal academy of higher education, Manipal, India
Department of Biotechnology, Manipal Institute of Technology Bengaluru, MAHE, Manipal, India
Department of Dental Materials, Manipal College of Dental Sciences, MAHE, Manipal, India
Department of Conservative Dentistry and Endodontics, KVG Dental College, Sullia, India
Department of Biotechnology, Manipal Institute of Technology Manipal, MAHE, Manipal, India
Department of Conservative Dentistry and Endodontics, Manipal College of Dental Sciences, Manipal academy of higher education, Manipal, India
چکیده
Partial or an incomplete penetration of bonding agent into the demineralized dentin can leave the denuded collagen fibres susceptible to degradation by photolytic enzymes. It is well known that the demineralized dentin and its collagen network is susceptible to degradation with time which can significantly affect the bond strength between root canal and sealer materials. The current study attempts assess the protective effect of proanthocyanidin (PA) loaded PLGA nanoparticles in minimizing the deterioration of demineralized dentin over a period. This is realized by determining/evaluating the push out bond strength between a glass fibre post cemented to root canal using resin luting cement. Ninety human mandibular premolars with single root canals were randomly divided into nine groups (n=۱۰) after endodontic treatment and post space preparation. The root canal dentin of the control group was left unconditioned and the experimental groups i.e. proanthocyanidin and PA loaded PLGA nanoparticles (PA-NP) were conditioned respectively with the test solutions. The glass fibre posts (GFP) were cemented into the root canal with dual cure resin cement (RC). The push out bond strength (POBS) was measured at ۳۰, ۶۰, and ۹۰ days. The mode of failure was evaluated with the help of stereomicroscope. The POBS were statistically analysed and evaluated with the help of paired t-test, ANOVA test and post hoc Bonferroni test at a confidence interval of ۹۵% (α = ۰.۰۵). Unloaded NP's average particle size was found to be ۲۳۷ ۱۶ nm, and its zeta potential was -۵۴±۴ mV. There was a statistically significant difference between the mean POBS at all time intervals among the groups tested. PA-NP showed a statistically significant higher bond strength compared to control at all time intervals, while it showed significantly better POBS compared to PA at ۱۸۰ days. All the experimental groups showed higher POBS at all intervals of time compared to control group. Application of natural cross-linkers such as PA improves POBS of GFP bonded to root canal dentin. PA loaded PLGA nanoparticles (PA-NP) as a conditioning liquid prior to root canal cementation not only improved POBS significantly, but also reduced the time dependent reduction in the bond strength.کلیدواژه ها
Nanoparticles, grape seed extract, Hybrid layer, PLGA, proanthocyanidin, Root canalاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.