Effect of Cigarette versus Electronic Cigarette on the Expression of TERT, FGFR۳, PTEN, P۵۳, and VEGF in Rat Bladder
- سال انتشار: 1402
- محل انتشار: فصلنامه تحقیقات بین رشته ای در اورولوژی، دوره: 5، شماره: 1
- کد COI اختصاصی: JR_TUMS-5-1_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 204
نویسندگان
School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Department of Biology, Khatam University, Tehran, Iran
Department of Biology, Medical Biotechnology Research Center, Yazd University, Yazd, Iran
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Department of Genetics, Medical Branch, Islamic Azad University, Tehran, Iran
AshianGanoTeb Biopharmaceutical Company, Golestan University of Medical Sciences, Gorgan, Iran
Department of Genetics & Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
Student Research Committee, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Pathology, University of California, Los Angeles, USA
Student Research Committee, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Introduction: This investigation aims to assess and compare the effect of cigarettes and e-cigarettes on the expression of TERT, FGFR۳, PTEN, P۵۳, and VEGF in rat bladder.Methods: ۶۰ Wistar rats were classified into three groups (۱۰): Group A (Control), Group B: case (cigarette smoke), and Group C: case (E-cigarette smoke). The rats were exposed to cigarettes or e-cigarettes for ۱۰ minutes. These ۱۰ minutes were performed three times a day, and in total, the samples were exposed to cigarettes or e-cigarettes for ۴۰ minutes a day with ۱-hour rest for ۱۶ weeks.Results: Histopathological findings showed cigarette-induced hyperplasia and e-cigarette-induced hyperemia and infiltration of inflammatory cells. The expression of FGFR۳, TERT, and VEGF genes significantly increased and the expression of the PTEN gene significantly decreased in both cigarette and e-cigarette groups in both male and female rats compared to the control group but these changes were not significant between the two groups. The expression of P۵۳ decreased in both groups, but the female rat in the e-cigarette significantly increased.Conclusion: We found that both groups changed the expression of genes involved in the development of BC, but no differences were found two groups. Therefore, the e-cigarette is not an excellent alternative to cigarettes.کلیدواژه ها
Bladder Cancer, E-cigarettes, Rat, Gene expressionاطلاعات بیشتر در مورد COI
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