Armeniacae Semen Regulates Apoptosis and Cell Cycle Progression in MCF-۷, MDA-MB-۲۳۱, and T۴۷D Breast Cancer Cell Lines
- سال انتشار: 1400
- محل انتشار: مجله سرطان خاورمیانه، دوره: 12، شماره: 2
- کد COI اختصاصی: JR_MISJ-12-2_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 196
نویسندگان
Department of Hematology and Blood Banking, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
Department of Hematology and Blood Banking, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
Department of Pharmacognosy and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Background: Amygdalin is a glycoside ingredient of rosacea plants that exerts an antitumor effect by blocking the growth of the tumor cells. Therefore, we aimed to use the Armeniacae semen, a member of the Rosacea family, which contains a large amount of the amygdalin, to evaluate its antiproliferative effect on MCF-۷, MDA-MB-۲۳۱, and T۴۷D breast cancer cell lines. Method: In this experimental study, we prepared the aqueous, ethyl acetate, and hydro-alcoholic extracts of the Armeniacae semen. The MCF-۷, MDA-MB-۲۳۱, and T۴۷D cell lines were treated with different doses of the extracts for ۱۲, ۲۴, ۳۶, and ۴۸ hours; cell viability was investigated with MTT test and cell apoptosis was detected by use of double staining fluorescent. Cell cycle progression was analyzed using a BD Cycle TEST PLUS DNA Kit. We also assessed Bcl۲, Bax, and caspase-۳ mRNA expression. Results: The best IC۵۰s belonged to hydro-alcoholic extract of the Armeniacae semen in all three cell lines for the ۴۸-hour treatment. We observed a significant increase in Bax and caspase-۳ mRNA expression and a noticeable reduction in Bcl۲ mRNA compared with the controls. Application of amygdalin to MCF-۷, MDA-MB-۲۳۱, and T۴۷D cell lines increased the number of G۰/G۱ cells and reduced the number of cells at G۲/M phasecompared to the controls. Conclusion: This study showed that the hydro-alcoholic extract of Prunus armeniaca had antitumor effects on breast cancer cell lines as it inhibited the cell cycle at G۰/G۱ phase and apoptosis induction in the MCF-۷, MDA-MB-۲۳۱, and T۴۷D cell lines.کلیدواژه ها
Armeniacae semen, Breast neoplasms, Cell Cycle, Caspase ۳, Proto-Oncogene Proteins c-bcl-۲, Baxاطلاعات بیشتر در مورد COI
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