The Kinetic Characteristics of Malic Enzyme in Human Breast Tissue Cancer Cell Lines MCF-۷ and MDA-MB-۲۳۱
- سال انتشار: 1398
- محل انتشار: مجله سرطان خاورمیانه، دوره: 10، شماره: 2
- کد COI اختصاصی: JR_MISJ-10-2_001
- زبان مقاله: انگلیسی
- تعداد مشاهده: 69
نویسندگان
Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Cancer Research Center (Petroleum and Environmental Pollutants), Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Cancer Research Center (Petroleum and Environmental Pollutants), Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده
Background: A high level of replication is one of the main indicators of tumors.Tumor cells have to manufacture and transport macromolecules into daughter cells. Oneof the required enzymes is malic enzyme, which generates the NADPH for fatty acidsynthesis in order to make cell membrane and pyruvate, and support the glycolysispathway to supply the energy demand. Due to the enormous proliferation of cancer cells,it is likely that the activity of malic enzyme in cancer cells is more than normal cells.The aim of this study is to survey the kinetics of malic enzyme in tumor and normalbreast tissues.Methods: We obtained the tumor and normal breast tissue specimens directlyfrom the operating room. The assays were performed with partially purified samplesunder optimum conditions for the substrate and co-factor requirements. The velocityof the enzyme or Michaelis-Menten constant, maximum velocity, and the amount ofinhibitor that reduced the enzyme activity by ۵۰% were obtained and calculated in allsamples.Results: The Michaelis-Menten constant for malate was lower in tumors comparedto normal samples. In contrast, the maximum velocity for malate in tumors was higherthan normal tissues, whereas the amount of inhibitor that reduced the enzyme activityby ۵۰% of guanidine hydrochloride and sodium chloride were both higher in tumorsthan normal tissues.Conclusion: The obtained results indicated that the malic enzyme kinetics haddifferent patterns in tumor tissues in comparison with normal tissues. A higher affinityof malic enzyme for pyruvate production in tumors supported high aerobic glycolysis.Moreover, it could be an approach to connect glutaminolysis to the glycolysis pathway.Malic enzyme could be a target to inhibit the glycolysis and glutaminolysis pathwaysin tumors.کلیدواژه ها
Malic enzyme, Breast cancer, Kinetics, Cancer metabolismاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.