Detection of Treponema phylotypes from digital dermatitis lesions and effect of different phylotypes on lesion size
- سال انتشار: 1401
- محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 13، شماره: 1
- کد COI اختصاصی: JR_VRFAN-13-1_011
- زبان مقاله: انگلیسی
- تعداد مشاهده: 199
نویسندگان
DVM Graduate, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده
Bovine digital dermatitis (BDD) is a contagious infectious disease which causes lameness in dairy cows. It has a multifactorial etiology which is not yet fully understood but Treponema spp. seem to play a significant role in development of BDD lesions. This study evaluated the presence of Treponema phylotypes commonly associated with BDD (T. medium/T. vincentii, T. phagedenis and T. putidum/T. denticola), in four farms different areas in Iran. Single biopsies were taken from ۱۱۳ Holstein cows with active BDD lesions (scored according to size) on the farms and polymerase chain reaction assays used to detect ۱۶S rRNA nucleotide fragments of three BDD Treponema phylotype groups: ‘‘T. medium/T. vincentii’’, ‘‘T. phagedenis’’ and ‘‘T. putidum/T. denticola’’ (now T. pedis). Over ۹۵.۰۰% of samples were positive for at least one of phylotypes, with ۸۹.۰۰%, ۹۱.۰۰ %, and ۶۶.۰۰% of samples were positive for T. putidum/T. denticola, T. phagedenis and T. medium/T. vincentii, respectively. Out of the ۱۱۳ samples, ۶۰.۰۰% were positive for all three phylotypes, the detection of T. putidum/T. denticola was positively associated with detection of both T. phagedenis and T. medium/T. vincentii. No association between lesion size and phylotypes identified was found but there were significant differences between farms in the proportion of each phylotypes identified. Further research is required to establish the factors influencing the proportions of individual phylotypes, especially at the farm level.کلیدواژه ها
Cow, Digital dermatitis, Lameness, Polymerase chain reaction (PCR), Treponemaاطلاعات بیشتر در مورد COI
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