First molecular report of Hydatigera krepkogorski (Schulz and Landa, ۱۹۳۴) in abdominal cavity of gerbil (Rhombomys opimus) in Iran

A polycephalic larva of Taeniidae family isolated from abdominal cavity of a great gerbil (Rhombomys opimus) from Golestan province, northern Iran, was subjected to molecular analysis. Genomic DNA from the larva was obtained using a DNA extraction tissue kit. Polymerase chain reaction was performed for amplification of the partial ۱۲S rRNA, cytochrome c oxidase subunit ۱ (cox۱) and NADH dehydrogenase ۱ (nad۱) mitochondrial genes. BLAST analysis of DNA sequencing indicated ۹۹.۰۰% homology in ۱۲S rRNA and cox۱ genes and ۹۸.۰۰% homology in nad۱ gene with Hydatigera krepkogorski (accession No. AB۷۳۱۷۶۲). The sequences of current isolate were deposited in GenBank by accession Nos. MF۲۸۱۹۷۱, MF۲۸۱۹۷۲ and MF۲۸۱۹۷۳ for ۱۲ SrRNA, cox۱ and nad۱ genes, respectively. This study was the first report of molecular characterization of H. krepkogorski from Iran. Isolation and characterization of the adult stage from definitive host will help to better clarify incomplete life cycle and morphology data of this species in the world.

کلیدواژه ها:

Hydatigera krepkogorski ، Iran ، Rhombomys opimus