Protective effects of Equisetum arvense methanolic extract on testicular tissue disorders in streptozotocin-induced diabetic murine model
- سال انتشار: 1400
- محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 12، شماره: 4
- کد COI اختصاصی: JR_VRFAN-12-4_016
- زبان مقاله: انگلیسی
- تعداد مشاهده: 89
نویسندگان
DVM Graduate, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
چکیده
Diabetes in a long period can damage the testicular tissue and impair the male fertility potential. Recently, different herbal treatments have been used for the prevention of type I diabetes and its pathological effects. Methanolic extract of Equisetum arvense has anti-oxidant and hypoglycemic properties. Thus, the current study aimed to evaluate the protective effects of Equisetum arvense methanolic extract (EE) on diabetes-induced detrimental effects in mice testicular tissue. Thirty-two adult male mice were randomly divided into four groups including control-sham, diabetic (induced by streptozotocin, ۵۰.۰۰ mg kg-۱ for five days), diabetic + EE ۲۵۰ (۲۵۰ mg kg-۱) and diabetic + EE ۵۰۰ (۵۰۰ mg kg-۱). After ۴۵ days, all animals were euthanized and their testicles were dissected out and undergone histological analyses. Moreover, the serum level of testosterone was evaluated. Analyses showed that seminiferous tubules diameter, Leydig cells number per mm۲ of the connective tissue, Sertoli cells number per tubule, serum level of testosterone and percentage of seminiferous tubules with positive tubular differentiation, repopulation and spermiogenesis indices were significantly decreased in the diabetic group in comparison with control-sham group. The administration of EE in test groups significantly decreased the adverse effects of diabetes (especially ۵۰۰ mg kg-۱). The results of this study revealed that diabetes disturbs spermatogenesis and spermiogenesis processes in mice. Meanwhile, the EE prevents diabetes-induced damages in mice testicular tissue, which may be associated with its hypoglycemic and antioxidative activities.کلیدواژه ها
Diabetes Mellitus, Field horsetail, Spermatogenesis, Spermiogenesis, Testosteroneاطلاعات بیشتر در مورد COI
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