Changes in the expression of OCT۴ in mouse ovary during estrous cycle
- سال انتشار: 1396
- محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 8، شماره: 1
- کد COI اختصاصی: JR_VRFAN-8-1_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 187
نویسندگان
Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran
Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran
Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran
Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
چکیده
The transcriptional factor OCT۴ regulates pluripotency of stem cells and has an important role during oocyte growth. Whereas, its role has remained ambiguous in ovarian tissue during reproductive cycle. Therefore, this study was aimed to investigate the expression patterns of OCT۴ in mouse ovaries during the normal estrous cycle. Adult National Medical Research Institute mice were classified as proestrous, estrous, metestrous and diestrous on the basis of vaginal smear cytology. Their ovaries were removed and the protein and gene expression levels of OCT۴ were assessed using immunohistochemical staining and real-time quantitative reverse-transcription PCR, respectively. Immunohistochemical staining revealed the expression of OCT۴ in the cytoplasm of corpus luteum cells. In the follicles, OCT۴ was expressed in the cytoplasm of granulosa cells. Furthermore, the gene expression levels of OCT۴ was significantly higher in the proestrous phase than in the other phases of the estrous cycle (p < ۰.۰۵). The results indicated that OCT۴ gene expression levels are affected by the cyclic pattern of the estrous cycle.کلیدواژه ها
Estrous cycle, Expression, Mouse, OCT۴, Ovarian tissueاطلاعات بیشتر در مورد COI
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