Phylogenetic typing and detection of extended-spectrum β-lactamases in Escherichia coli isolates from broiler chickens in Ahvaz, Iran
- سال انتشار: 1395
- محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 7، شماره: 3
- کد COI اختصاصی: JR_VRFAN-7-3_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 192
نویسندگان
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Post Graduate Student, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Department of Microbiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده
This study was conducted to reveal the phylogenetic background, to detect the genes encoding TEM, SHV and CTX-M-۱۵ extended-spectrum β-lactamases (ESBL), and to analyze their distribution in phylo-groups of ۱۵۰ Escherichia coli isolates from broiler chickens in Ahvaz (Southwest of Iran). Seventy- five cloacal swabs from healthy birds (fecal isolates), and ۷۵ heart blood samples from birds with colibacillosis (septicemic isolates) were obtained. All isolates were phylotyped and screened for ESBL genes by polymerase chain reaction (PCR). The fecal isolates belonged to four main phylo-groups, including ۴۱ isolates (۵۴.۶۷%) to A, ۹ (۱۲.۰۰%) to B۱, ۵ (۶.۶۷%) to B۲, and ۲۰ (۲۶.۶۷%) to D. Of septicemic isolates, ۳۷ isolates (۴۹.۳۳%) were classified as phylotype A, ۵ (۶.۶۷%) as B۱, ۱۰ (۱۳.۳۳%) as B۲, and ۲۳ (۳۰.۶۷%) as D. In molecular analysis, a total of ۷۲ isolates (۳۵ fecal and ۳۷ septicemic) were identified to harbor ESBL genes, which were distributed in phylo-groups A, B۱, B۲, and D. Regardless of the type of isolate, blaCTX-M-۱۵ gene was the most common genotype, followed by blaTEM and blaSHV genes. This study suggests that broiler chickens in Iran are infected to ESBL genes- harboring Escherichia coli strains which may be spread to the food chain through fecal contamination of carcasses during slaughtering.کلیدواژه ها
beta-Lactamase, Chicken, Escherichia coli, phylogenyاطلاعات بیشتر در مورد COI
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