Characterization of Morphological, Phytochemical and Molecular Diversity of Artemisia annua Accessions in Hyrcanian Area of Iran

  • سال انتشار: 1398
  • محل انتشار: مجله علوم و فناوری کشاورزی، دوره: 21، شماره: 5
  • کد COI اختصاصی: JR_JASTMO-21-5_016
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 55
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نویسندگان

M. R. Naghavi

Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Islamic Republic of Iran.

E. Aryakia

Iranian Biological Resource Center (IBRC), (ACECR), Karaj, Islamic Republic of Iran.

S. Hadi

Department of Horticulture, Biotechnology and Molecular Genetics, College of Agriculture and Natural Resources, Karaj Islamic Azad University, Alborz, Islamic Republic of Iran.

Hossein Ghafoori

Department of Biology, University of Guilan, Rasht, Islamic Republic of Iran.

H. Mousavi

Iranian Biological Resource Center (IBRC), (ACECR), Karaj, Islamic Republic of Iran.

H. Ramazani

Iranian Biological Resource Center (IBRC), (ACECR), Karaj, Islamic Republic of Iran.

M. Feyzbakhsh

Iranian Biological Resource Center (IBRC), (ACECR), Karaj, Islamic Republic of Iran.

Y. Ajani

Iranian Biological Resource Center (IBRC), (ACECR), Karaj, Islamic Republic of Iran.

Z. Farahmand

Iranian Biological Resource Center (IBRC), (ACECR), Karaj, Islamic Republic of Iran.

L. Poorhosseini

Department of Horticulture, Biotechnology and Molecular Genetics, College of Agriculture and Natural Resources, Karaj Islamic Azad University, Alborz, Islamic Republic of Iran.

S. A. Shahzadeh Fazeli

Iranian Biological Resource Center (IBRC), (ACECR), Karaj, Islamic Republic of Iran.

چکیده

Artemisia annua L. is an important medicinal plant used as an original source of artemisinin for treating malaria. Although there is a wide distribution of A. annua in Hyrcanian Areas (Mazandaran, Guilan, and Golestan Provinces in N Iran), no considerable effort has been made for diversity assessment. In this study, morphological, phytochemical, and molecular characterization of A. annua accessions in this area were assessed using ۴ quantitative characteristics (height, fresh weight, dry weight, and trichome density), artemisinin content, and ۱۵ ISSR primers. Using these traits, a high level of morphological, phytochemical and molecular diversity was revealed among A. annua accessions in the provinces (populations) of Hyrcanian Areas. At inter-population level, the highest value of artemisinin was observed in Mazandaran Province. Moreover, significant correlation between artemisinin content and trichome density was observed that could be useful for indirect selection of artemisinin yield in different accessions of A. annua. In addition, fifteen ISSR primers generated a total number of ۲۲۲ amplified bands, consisting of ۱۷۷ and ۴۵ polymorphic and monomorphic loci across the ۶۰ accessions, respectively. These results indicate that ISSR-PCR is a reliable tool for fingerprinting A. annua at the intra-population level. Our results altogether are valid contributions for gene bank management and Artemisia annua breeding programs.

کلیدواژه ها

A. annua, Artemisinin, Breeding, ISSR, UPGMA., تنوع, مناطق هیرکانی, گندواش, آرتمیزینین

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