Combination of Cry۱Ac and Cry۲A to Produce Resistance against Helicoverpa armigera in Cotton
- سال انتشار: 1399
- محل انتشار: مجله علوم و فناوری کشاورزی، دوره: 22، شماره: 6
- کد COI اختصاصی: JR_JASTMO-22-6_014
- زبان مقاله: انگلیسی
- تعداد مشاهده: 199
نویسندگان
Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
Centre of Excellence in Molecular Biology, university of the Punjab, Lahore, Pakistan
Centre of excellence in molecular biology, University of the Punjab, Lahore, Pakistan
Centre of Excellence in Molecular Biology, university of the Punjab, Lahore, Pakistan
Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
چکیده
Genetic manipulation to get desirable characters in cash crops like cotton remains the prime objective of crop biotechnology. To produce transgenic plants resistant against bollworms, newly emerged embryos of MNH-۷۸۶ cotton variety were transformed with Agrobacterium tumefaciens strain LBA۴۴۰۴ harboring the plasmid pKHG۴ vector containing Cry۱Ac+Cry۲A genes under the control of CaMV۳۵S promoter. The integration and expression of these genes were evaluated by PCR, Florescent In-Situ Hybridization (FISH), and ELISA, respectively. Out of ۷۰۰ putative transgenic cotton plants, ۱۰ plants (۱.۰۳% transformation efficiency) showed the presence of genes Cry۱Ac+Cry۲A through PCR analysis. In vitro, insect feeding bioassay was done for estimation of mortality percentage of Helicoverpa armigera. Insect mortality rate and morphological characteristics of Bt cotton were analyzed by phenotypic correlation, path coefficient regression and covariance to evaluate the advantage of transgenic technology in numerical terms. Statistical analysis indicated significant positive correlation between insect mortality and cotton seed yield. Helicoverpa armigera mortality data produced a directly proportional relation with cotton seed yield. The results of this study support the improvement of cotton defense mechanism against insects and natural competitors through genetic modification.کلیدواژه ها
Bollworms, Insect mortality, Transgenic cotton.اطلاعات بیشتر در مورد COI
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