Metformin and Pioglitazone Reduce Gene Expression of Inflammatory Factors in Insulin Resistant and Hypertrophied Adipocytes
- سال انتشار: 1399
- محل انتشار: مجله دیابت و چاقی ایران، دوره: 12، شماره: 4
- کد COI اختصاصی: JR_IJDO-12-4_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 74
نویسندگان
Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Department of Genetics, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
چکیده
Objective: In obesity, chronic low grade inflammation, created by induction of pro-inflammatory markers, causes adipocyte dysfunction in adipose tissue. Adipocytes dysfunction is associated with various diseases including insulin resistance and obesity. In obesity, inflammatory factors such as osteopontin (OPN), angiopoietin-like protein ۲ (Angptl۲) and transforming growth factor-β (TGF-β) are induced in adipose tissue. Metformin and pioglitazone that are used to modulate inflammation, but the relevant mechanism is poorly understood. This study aimed to investigate the effect of metformin and pioglitazone as anti-diabetic drugs, on gene expression of osteopontin, Angptl۲ and TGF-β as inflammatory factors in insulin resistance and hypertrophied adipocyte in ۳T۳-L۱ cell line model in vitro. Materials and Methods: In this experimental research, we differentiated۳T۳-L۱ preadipocytes to adipocytes. The adipocytes treated in insulin resistance and hypertrophied conditions with metformin and pioglitazone and assayed gene expression of OPN, Angptl۲ and TGF-β by Real-Time PCR. Data was analyzed by SPSS statistic software. Results: The results showed that expression of OPN, Angptl۲, and TGF-β were increased significantly over ۲-fold (P-value< ۰.۰۵) in insulin resistance and hypertrophied adipocytes compared to normal adipocytes. Pre- and co-treatment with metformin and pioglitazone led to reduced expression of Angptl۲ and TGF-β. Only metformin significantly reduced the expression of Angptl۲, TGF-β and OPN in hypertrophied adipocyte. Conclusion: These results support the proposal that metformin and pioglitazone reduce gene expression of inflammatory factors in insulin resistant and hypertrophied adipocytes.کلیدواژه ها
Angptl۲, metformin, Osteopontin, Pioglitazone, TGF-βاطلاعات بیشتر در مورد COI
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