Bacterial pathogens associated with citrus huanglongbing-like symptoms in southern Iran
- سال انتشار: 1395
- محل انتشار: Journal of Crop Protection، دوره: 6، شماره: 1
- کد COI اختصاصی: JR_JCP-6-1_010
- زبان مقاله: انگلیسی
- تعداد مشاهده: 168
نویسندگان
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Plant Protection Research Department, South Kerman Agricultural and Natural Resources Research Centre, AREEO, Jiroft, Iran.
Plant Protection Research Department, South Kerman Agricultural and Natural Resources Research Centre, AREEO, Jiroft, Iran.
چکیده
Huanglongbing (HLB) also known as citrus greening, is a destructive disease of citrus and now, is considered as a new emergence and spread out threat to the Middle East and North Africa (MENA) citrus production. In a survey conducted in southern Iran in ۲۰۱۳-۲۰۱۴, ۷۷ citrus samples exhibiting symptoms of HLB were collected. Single-step and nested polymerase chain reactions (PCR) were employed to determine the presence of the phloem-limited bacterial pathogens ‘Candidatus Liberibacter asiaticus’(CLas), phytoplasma and Spiroplasma citri. Both CLas and phytoplasma were detected in HLB-affected citrus trees as co-infection (۷.۷۹%) and single infection (۱۰.۳۸% for phytoplasma and ۴۲.۸۵% for CLas). According to the proposed ۱۶S rDNA-based phytoplasma classification scheme, the HLB-associated phytoplasma from this study was a member of peanut witches’broom (۱۶SrII) phytoplasma group. This is the first report of association of a phytoplasma with HLB in sweet lime in the world and first record of association of CLas with sour orange (Citrus aurantium L.) and sweet lime in Iran.کلیدواژه ها
'Candidatus Liberibacter asiaticus', Citrus greening, Phytoplasma, Citrus, Iranاطلاعات بیشتر در مورد COI
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